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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Overview
Product name
Anti-Thrombospondin antibody [A4.1]
See all Thrombospondin products (11) ...
Description
Mouse monoclonal [A4.1] to Thrombospondin
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Tested applications
Flow Cyt, IF, WB, Functional Studiesmore details
Cross reactivity
Reacts with
Cow, Human
Predicted to work with
Mouse, Rat, Chicken
Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Positive control
HUVEC cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purification notes
Purified from ascites fluid by ammonium sulfate precipitation.
Clonality
Monoclonal
Clone number
A4.1
Isotype
IgM
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 450 kDa.
FuncS: Use at an assay dependent dilution. (Blocks the anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36 and inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1.)
Target
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
Target information above from: UniProt accessionP07996
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- THBS 1 antibody
- THBS antibody
- THBS1 antibody
see all
Database links
- Entrez Gene: 373987 Chicken
- Entrez Gene: 281530 Cow
- Entrez Gene: 7057 Human
- Entrez Gene: 21825 Mouse
- Entrez Gene: 445442 Rat
- Omim: 188060 Human
- SwissProt: Q28178 Cow
- SwissProt: P07996 Human
see all
References for Anti-Thrombospondin antibody [A4.1] (ab3131)
ab3131 has not yet been referenced specifically in any publications.
Publishing research using ab3131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"