Thrombospondin antibody [A4.1] (ab3131)

Product Name 

Thrombospondin antibody [A4.1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [A4.1] to Thrombospondin

Immunogen 

Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow

Specificity 

It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.

Epitope 

N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.

Tested applications 
(see key)

Flow Cyt, FuncS, ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Flow Cytometry / Immunofluorescence: assay dependent.
Western Blotting (Non-reduced only): use at a concentration of 1 - 2µg/ml for 2hrs at RT.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects a band of ~ 450 kDa, which corresponds to the predicted molecular weight of Thrombospondin.

This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36.

This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1.

It does not inhibit aggregation of thrombin-induced platelets.

Positive control 
(see definition)

HUVEC cells.

Cellular localization 

Cell Membrane

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

Relevance 

Thrombospondin is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Thrombospondin also has multiple binding sites that interact with molecules such as fibronectin, collagens, laminin and heparan sulphate proteoglycans as well as binding growth factors such as TGF-beta1. Thrombospondin exerts an anti-adhesive effect which leads to cell rounding and detachment. Thrombospondin is also implicated in a key role in brain development and synapse regulation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

A4.1

Isotype 

IgM

Light chain type 

kappa

Storage buffer 

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites fluid by ammonium sulfate precipitation.

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

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