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ab1823 has been referenced in 12 publications.
Publishing research using ab1823? Please let us know so that we can cite the reference in this datasheet
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ab1823 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with Thrombospondin, using ABC and DAB chromogen.
ab1823 at a 1/25 dilution staining Human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following head mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20ºC. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20ºC. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
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