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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)](/ps/datasheet/images/ab1823_1.jpg)
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)](/ps/datasheet/images/ab1823_2.jpg)
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Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)
![Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)](\ps\datasheet\images\1\ab1823\Thrombospondin-Primary-antibodies-ab1823-7.jpg)
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Product Name
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Thrombospondin antibody [A6.1]
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See all Thrombospondin antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [A6.1] to Thrombospondin
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Immunogen
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BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Dog, Hrs, Pig, Shp
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Specificity
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This antibody is specific to the 450 kDa protein, a non-reduced form, and 170 to 180 kDa protein, a reduced form, of Thrombospondin.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tonsil
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Cellular localization
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Cell Membrane
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
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Relevance
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Thrombospondin is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Thrombospondin also has multiple binding sites that interact with molecules such as fibronectin, collagens, laminin and heparan sulphate proteoglycans as well as binding growth factors such as TGF-beta1. Thrombospondin exerts an anti-adhesive effect which leads to cell rounding and detachment. Thrombospondin is also implicated in a key role in brain development and synapse regulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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A6.1
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Thrombospondin antibody [A6.1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Thrombospondin antibody [A6.1] images, references, products related to ab1823 and other tools.
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Thrombospondin antibody [A6.1] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)
ab1823 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with Thrombospondin, using ABC and DAB chromogen.
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)
ab1823 at a 1/25 dilution staining Human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following head mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe . This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
See Abreview
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Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)
ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)
![Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)](/ps/datasheet/images/1/ab1823/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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References for Thrombospondin antibody [A6.1] (ab1823)
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This product has been used in: (two most recent references)
Komuro A et al. Diffuse-type gastric carcinoma: progression, angiogenesis, and transforming growth factor beta signaling. J Natl Cancer Inst 101:592-604 (2009). IHC-P; Mouse. PubMed: 19351925
Thulin A et al. Activated platelets provide a functional microenvironment for the antiangiogenic fragment of histidine-rich glycoprotein. Mol Cancer Res 7:1792-802 (2009). PubMed: 19903770
See all 13 publication references for this product.
If you publish research using ab1823 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Thrombospondin
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Thrombospondin antibody [A6.1] - more information
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Customer reviews (feedback) regarding Thrombospondin antibody [A6.1] |
Customer FAQs regarding Thrombospondin antibody [A6.1] |
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Thrombospondin antibody [A6.1] - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)](/index.html?datasheet=1823#ab1823_1.jpg)
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