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Anti-Thrombospondin antibody [A6.1] (ab1823)

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Overview

Product name

Anti-Thrombospondin antibody [A6.1]
See all Thrombospondin products (11) ...

Description

Mouse monoclonal [A6.1] to Thrombospondin

Specificity

This antibody is specific to the 450 kDa protein, a non-reduced form, and 170 to 180 kDa protein, a reduced form, of Thrombospondin.

Tested applications

ICC/IF, IHC-P, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig

Immunogen

BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Positive control

Tonsil

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

A6.1

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823) image (enlarge)

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.

Sequence similarities

Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.

Target information above from: UniProt accessionP07996 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • THBS 1 antibody
  • THBS antibody
  • THBS1 antibody
  • Thrombospondin 1 antibody
  • Thrombospondin-1 antibody
  • Tsp 1 antibody
  • TSP antibody
  • Tsp1 antibody
  • TSP1_HUMAN antibody
see all

Database links

see all

Anti-Thrombospondin antibody [A6.1] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)

ab1823 - immunohistochemistry

Formalin fixed paraffin embedded human tonsil stained with Thrombospondin, using ABC and DAB chromogen.

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)

Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Thrombospondin antibody [A6.1] (ab1823)

ab1823 at a 1/25 dilution staining Human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following head mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .

This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.

See Abreview

  Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)

Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)

ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)

Immunocytochemistry/ Immunofluorescence - Thrombospondin antibody [A6.1] (ab1823)

ab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-Thrombospondin antibody [A6.1] (ab1823)

This product has been referenced in:

  • Banerjee Aet al. Unfolded Protein Response Is Required in nu/nu Mice Microvasculature for Treating Breast Tumor with Tunicamycin. J Biol Chem 286:29127-38 (2011). WB; Mouse.Read more (PubMed: 21676868) »
  • Guo Wet al. Aberrant methylation of thrombospondin-1 and its association with reduced expression in gastric cardia adenocarcinoma. J Biomed Biotechnol 2010:721485 (2010). IHC-P; Human.Read more (PubMed: 20300551) »

See all 12 publications for this product

Publishing research using ab1823? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"