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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Overview
Product name
Anti-Thrombospondin antibody [TSP-B7]
See all Thrombospondin products (11) ...
Description
Mouse monoclonal [TSP-B7] to Thrombospondin
Specificity
The antibody does not cross react with human thrombin, fibrinogen, fibronectin, laminin or serum albumin. Cross-reactivity with fetal calf serum has been observed, therefore the presence of fetal calf serum in an assay system may interfere with antibody activity.
Tested applications
ELISA, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Thrombin induced platelet releasate (Human).
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
TSP-B7
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
IP
Application notes
ELISA: 1/1000. This concentration was determined using thrombin-induced platelet releasate as the coat (1:250 dilution of releasate from 1 unit of thrombocyte concentrate/ml of PBS).
IP: Use at an assay dependent dilution. This technique can be used with heat-killed, fixed suspensions of Staphylococcus aureus or Sepharose-Protein A.
WB: Use at an assay dependent dilution. Predicted molecular weight of protein in native state: 420 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
Target information above from: UniProt accessionP07996
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- THBS 1 antibody
- THBS antibody
- THBS1 antibody
see all
Database links
- Entrez Gene: 7057 Human
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
References for Anti-Thrombospondin antibody [TSP-B7] (ab11579)
This product has been referenced in:
- Babiker AAet al. Mapping pro- and antiangiogenic factors on the surface of prostasomes of normal and malignant cell origin. Prostate 70:834-47 (2010).Read more (PubMed: 20127731) »
- Bäck Jet al. Distinctive regulation of contact activation by antithrombin and C1-inhibitor on activated platelets and material surfaces. Biomaterials 30:6573-80 (2009). ELISA; Human.Read more (PubMed: 19783299) »
See all 2 publications for this product
Publishing research using ab11579? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"