Thrombospondin antibody [TSP-B7] (ab11579)

Product Name 

Thrombospondin antibody [TSP-B7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [TSP-B7] to Thrombospondin

Immunogen 

Thrombin induced platelet releasate (Human).

Reacts with 
(species key)

Hu

Specificity 

The antibody does not cross react with human thrombin, fibrinogen, fibronectin, laminin or serum albumin.
Cross-reactivity with fetal calf serum has been observed, therefore the presence of fetal calf serum in an assay system may interfere with antibody activity.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000. This concentration was determined using thrombin-induced platelet releasate as the coat (1:250 dilution of releasate from 1 unit of thrombocyte concentrate/ml of PBS).
IP: Use at an assay dependent dilution. This technique can be used with heat-killed, fixed suspensions of Staphylococcus aureus or Sepharose-Protein A.
WB: Use at an assay dependent dilution. Predicted molecular weight of protein in native state: 420 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic inhibitory factors
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Regulation
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

Relevance 

Thrombospondin is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Thrombospondin also has multiple binding sites that interact with molecules such as fibronectin, collagens, laminin and heparan sulphate proteoglycans as well as binding growth factors such as TGF-beta1. Thrombospondin exerts an anti-adhesive effect which leads to cell rounding and detachment. Thrombospondin is also implicated in a key role in brain development and synapse regulation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

TSP-B7

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 0.1% Sodium Azide.
Constituents: Ascites fluid.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

10.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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