Anti-Thrombospondin 1 antibody (ab88529)
Key features and details
- Mouse polyclonal to Thrombospondin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Thrombospondin 1 antibody
See all Thrombospondin 1 primary antibodies -
Description
Mouse polyclonal to Thrombospondin 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Thrombospondin 1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human pancreas tissue lysate and Thrombospondin transfected 293T cell lysate IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
Cellular localization
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 7057 Human
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
- Unigene: 732539 Human
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Alternative names
- Thbs1 antibody
- Thrombospondin-1 antibody
- TSP antibody
see all
Images
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All lanes : Anti-Thrombospondin 1 antibody (ab88529) at 1 µg/ml
Lane 1 : Thrombospondin transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 129 kDa -
ab88529 at 10 µg/ml staining Thrombospondin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
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Anti-Thrombospondin 1 antibody (ab88529) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 129 kDa
Observed band size: ~ 150 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab88529 has been referenced in 6 publications.
- Guo L et al. Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. Theranostics 10:6581-6598 (2020). PubMed: 32550891
- Liu Y et al. MicroRNA-182-5p contributes to the protective effects of thrombospondin 1 against lipotoxicity in INS-1 cells. Exp Ther Med 16:5272-5279 (2018). PubMed: 30546417
- Duan S et al. Effects of taxol resistance gene 1 on the cisplatin response in gastric cancer. Oncol Lett 15:8287-8294 (2018). PubMed: 29805561
- Patel GK et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). PubMed: 28152544
- Xu C et al. Circadian Clock Regulates Bone Resorption in Mice. J Bone Miner Res 31:1344-55 (2016). PubMed: 26841172
- Bonazzi VF et al. Cross-Platform Array Screening Identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as Genes Frequently Silenced by Methylation in Melanoma. PLoS One 6:e26121 (2011). WB ; Human . PubMed: 22028813