![Flow Cytometry - Thy1.2 antibody [30-H12] (FITC) (ab24905) image](#ab24905_1.jpg)
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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-Thy1.2 antibody [30-H12] (FITC)
See all Thy1.2 products (9) ...
Description
Rat monoclonal [30-H12] to Thy1.2 (FITC)
Conjugation
FITC
Specificity
ab24905 does not cross react with strains bearing the Thy 1.1 alloantigen (e.g., AKR/J, PL).
Tested applications
Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
The details of the immunogen for this antibody are not available.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
30-H12
Isotype
IgG2b
Light chain type
kappa
Research areas
-
Flow Cytometry - Thy1.2 antibody [30-H12] (FITC) (ab24905)
![Flow Cytometry - Thy1.2 antibody [30-H12] (FITC) (ab24905) image](/ps/datasheet/Images/24/ab24905/ab24905_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
Flow Cyt
IHC-Fr
Application notes
Flow Cyt: Use at an assay dependent dilution. Use up to 1ug/106 cells.
IHC-Fr: Use at an assay dependent dilution.
In vivo and in vitro depletion of T cells: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
CD90/Thy 1 is a GPI anchored molecule and one of the smallest members of the immunoglobulin superfamily, it contains a single V type (immunoglobulin like) domain. The Thy 1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non lymphoid tissues. Thy 1.2 may play a role in cell cell or cell ligand interactions during synaptogenesis and other events in the brain.
Cellular localization
Cell Membrane; attached to the membrane by a GPI anchor.
Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
Anti-Thy1.2 antibody [30-H12] (FITC) images:
Flow Cytometry - Thy1.2 antibody [30-H12] (FITC) (ab24905)
BALB/c splenocytes were double-stained with ab24905 (0.3 ug/106 cells) and Rat anti-Mouse CD19R-PE. Small lymphocytes were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
References for Anti-Thy1.2 antibody [30-H12] (FITC) (ab24905)
This product has been referenced in:
- Williams AF & Gagnon J Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science 216:696-703 (1982).Read more (PubMed: 6177036) »
- Ledbetter JAet al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980).Read more (PubMed: 6156984) »
See all 3 publications for this product
Publishing research using ab24905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"