![Immunohistochemistry (Frozen sections) - Thy1.2 antibody [30-H12] (ab25593) image](#Thy12-Primary-antibodies-ab25593-4.jpg)
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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-Thy1.2 antibody [30-H12]
See all Thy1.2 products (9) ...
Description
Rat monoclonal [30-H12] to Thy1.2
Specificity
This antibody does not cross react with mouse and rat strains bearing the Thy1.1 alloantigen.
Tested applications
Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
The details of the immunogen for this antibody are not available.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 100mM Borate buffered saline, pH 8.2
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
This antibody has been shown to be useful in 'in vivo' and 'in vitro' studies of T cell depletion.
Clonality
Monoclonal
Clone number
30-H12
Isotype
IgG2b
Light chain type
kappa
Research areas
-
Immunohistochemistry (Frozen sections) - Thy1.2 antibody [30-H12] (ab25593)
![Immunohistochemistry (Frozen sections) - Thy1.2 antibody [30-H12] (ab25593) image](/ps/datasheet/images/25/ab25593/Thy12-Primary-antibodies-ab25593-4.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab25593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Target
Relevance
CD90/Thy 1 is a GPI anchored molecule and one of the smallest members of the immunoglobulin superfamily, it contains a single V type (immunoglobulin like) domain. The Thy 1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non lymphoid tissues. Thy 1.2 may play a role in cell cell or cell ligand interactions during synaptogenesis and other events in the brain.
Cellular localization
Cell Membrane; attached to the membrane by a GPI anchor.
Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
Anti-Thy1.2 antibody [30-H12] images:
Immunohistochemistry (Frozen sections) - Thy1.2 antibody [30-H12] (ab25593)
ab225593 staining Thy1.2 in murine brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at 4°C. An Alexa Fluor®594-conjugated donkey anti-rat polyclonal was used as secondary antibody at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Thy1.2 antibody [30-H12] (ab25593)
This product has been referenced in:
- Koilkonda RDet al. Induction of rapid and highly efficient expression of the human ND4 complex I subunit in the mouse visual system by self-complementary adeno-associated virus. Arch Ophthalmol 128:876-83 (2010).Read more (PubMed: 20625049) »
- Ettaiche Met al. Acid-sensing ion channel 3 in retinal function and survival. Invest Ophthalmol Vis Sci 50:2417-26 (2009).Read more (PubMed: 19117938) »
See all 3 publications for this product
Publishing research using ab25593? Please let us know so that we can cite the reference in this datasheet
![Thy1.2 antibody [30-H12] for Immunohistochemistry (Frozen sections) in Mouse (25593)](/ps/datasheet/images/25/ab25593/Thy12-Primary-antibodies-ab25593-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"