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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-Thy1.2 antibody [FF-10]
See all Thy1.2 products (9) ...
Description
Rat monoclonal [FF-10] to Thy1.2
Specificity
ab22489 recognises Thy1.2 cell surface antigen, and binds to T cell lines (EL 4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.
Tested applications
Flow Cyt, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Tissue / cell preparation (Mouse): EL-4 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
FF-10
Myeloma
Sp2/0-Ag14
Isotype
IgG2c
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
Flow Cyt: 1/50 - 1/100.Flow Cyt: 1/50 - 1/100.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 30 kDa.
Target
Relevance
CD90/Thy 1 is a GPI anchored molecule and one of the smallest members of the immunoglobulin superfamily, it contains a single V type (immunoglobulin like) domain. The Thy 1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non lymphoid tissues. Thy 1.2 may play a role in cell cell or cell ligand interactions during synaptogenesis and other events in the brain.
Cellular localization
Cell Membrane; attached to the membrane by a GPI anchor.
Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
References for Anti-Thy1.2 antibody [FF-10] (ab22489)
This product has been referenced in:
- Fan Xet al. ICAM-1-, -2- and LFA-1-independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule. Inflammation 20:401-11 (1996).Read more (PubMed: 8872503) »
See 1 publication for this product
Publishing research using ab22489? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"