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Product Name
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Thy1.2 antibody [FF-10]
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See all Thy1.2 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [FF-10] to Thy1.2
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Immunogen
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Tissue / cell preparation (Mouse): EL-4 cells
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Reacts with
(species key)
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Ms
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Specificity
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ab22489 recognises Thy1.2 cell surface antigen, and binds to T cell lines (EL 4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50 - 1/100.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 30 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; attached to the membrane by a GPI anchor.
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Research areas
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Immunology >> Immunoglobulins >> Receptors
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Relevance
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CD90/Thy 1 is a GPI anchored molecule and one of the smallest members of the immunoglobulin superfamily, it contains a single V type (immunoglobulin like) domain. The Thy 1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non lymphoid tissues. Thy 1.2 may play a role in cell cell or cell ligand interactions during synaptogenesis and other events in the brain.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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FF-10
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG2c
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Thy1.2 antibody [FF-10] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Thy1.2 antibody [FF-10] (ab22489)
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This product has been used in:
Fan X et al. ICAM-1-, -2- and LFA-1-independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine Thy-1 molecule. Inflammation 20:401-11 (1996). PubMed: 8872503
If you publish research using ab22489 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Thy1.2
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Thy1.2 antibody [FF-10] - more information
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