![Western blot - Thymidine Phosphorylase antibody [P-GF.44C] (ab3151) image](#Thymidine-Phosphorylase-Primary-antibodies-ab3151-3.jpg)
 image](#Thymidine-Phosphorylase-Primary-antibodies-ab3151-4.jpg)
![Immunoprecipitation - Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab3151) image](#Thymidine-Phosphorylase-Primary-antibodies-ab3151-5.jpg)
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Product name
Anti-Thymidine Phosphorylase antibody [P-GF.44C]
See all Thymidine Phosphorylase products (3) ...
Description
Mouse monoclonal [P-GF.44C] to Thymidine Phosphorylase
Tested applications
IHC-Fr, Neutralising, IP, Flow Cyt, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Recombinant full length human thymidine phosphorylase (TP / PD-ECGF) protein.
Positive control
HUVEC cells. Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
Platelet - derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.
Clonality
Monoclonal
Clone number
P-GF.44C
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Signal Transduction >> Growth Factors/Hormones >> FGF
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
Neut: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
IHC-P: Use a concentration of 1 - 2 µg/ml. No special pretreatment is required for immuno-histochemical staining of formalin-fixed tissues.
WB: Use at an assay dependent dilution. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).
Target
Relevance
In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.
Cellular localization
intracellular organelle membrane
Alternative names
- ECGF 1 antibody
- ECGF1 antibody
- Endothelial cell growth factor 1 antibody
see all
Database links
- Entrez Gene: 1890 Human
- Entrez Gene: 72962 Mouse
- Entrez Gene: 315219 Rat
- Omim: 131222 Human
- SwissProt: P19971 Human
- SwissProt: Q99N42 Mouse
- SwissProt: Q9D6E8 Mouse
- SwissProt: Q8VCL2 Mouse
see all
Anti-Thymidine Phosphorylase antibody [P-GF.44C] images:
Western blot - Thymidine Phosphorylase antibody [P-GF.44C] (ab3151)
![Western blot - Thymidine Phosphorylase antibody [P-GF.44C] (ab3151)](/ps/datasheet/images/3/ab3151/Thymidine-Phosphorylase-Primary-antibodies-ab3151-3.jpg)
All lanes : Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab3151) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
Flow Cytometry-Thymidine Phosphorylase antibody [P-GF.44C](ab3151)
](/ps/datasheet/images/3/ab3151/Thymidine-Phosphorylase-Primary-antibodies-ab3151-4.jpg)
Overlay histogram showing A431 cells stained with ab3151 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3151, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunoprecipitation - Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab3151)
![Immunoprecipitation - Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab3151)](/ps/datasheet/images/3/ab3151/Thymidine-Phosphorylase-Primary-antibodies-ab3151-5.jpg)
Thymidine Phosphorylase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Thymidine Phosphorylase (ab3151)and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab3151. Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Bands: 52kDa:Thymidine Phosphorylase
References for Anti-Thymidine Phosphorylase antibody [P-GF.44C] (ab3151)
This product has been referenced in:
- Ait-Tihyaty M et al. Inhibition of EGFR phosphorylation in a panel of human breast cancer cells correlates with synergistic interactions between gefitinib and 5'-DFUR, the bioactive metabolite of Xeloda(®). Breast Cancer Res Treat : (2011).Read more (PubMed: 21915635) »
- Coffelt SB et al. Angiopoietin-2 regulates gene expression in TIE2-expressing monocytes and augments their inherent proangiogenic functions. Cancer Res 70:5270-80 (2010). WB, IHC-Fr; Human, Mouse.Read more (PubMed: 20530679) »
See all 5 publications for this product
Publishing research using ab3151? Please let us know so that we can cite the reference in this datasheet
![Thymidine Phosphorylase antibody [P-GF.44C] for WB in Human (3151)](/ps/datasheet/images/3/ab3151/Thymidine-Phosphorylase-Primary-antibodies-ab3151-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"