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Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)

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Overview

  • Product nameThymidylate Synthase 293T Transfected Lysate - (positive control)
  • General notesab94158 is a 293T cell transfected lysate in which Human Thymidylate Synthase has been transiently over-expressed using a pCMV-Thymidylate Synthase plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Pathway: Pyrimidine metabolism; dTTP biosynthesis. Similarity: Belongs to the thymidylate synthase family.

    • Applications

    Our Abpromise guarantee covers the use of ab94158 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Thymidylate Synthase 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)
      SDS-PAGE - Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)
      ab94158 at 15 µg/lane on an SDS-PAGE gel
    • Western blot - Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)
      Western blot - Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)
      All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1/500 dilution

      Lane 1 : Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)
      Lane 2 : 293T non-transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Thymidylate Synthase 293T Transfected Lysate - (positive control) (ab94158)

    ab94158 has not yet been referenced specifically in any publications.

    Publishing research using ab94158 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"