![Western blot - Thymidylate Synthase antibody [TS 106] (ab3145) image](#ab3145_1.jpg)
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Product name
Anti-Thymidylate Synthase antibody [TS 106]
See all Thymidylate Synthase products (9) ...
Description
Mouse monoclonal [TS 106] to Thymidylate Synthase
Tested applications
Flow Cyt, IF, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Positive control
5-FU-resistant cell cancer lines or colonic carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
TS 106
Isotype
IgG1
Light chain type
kappa
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Western blot - Thymidylate Synthase antibody [TS 106] (ab3145)
![Western blot - Thymidylate Synthase antibody [TS 106] (ab3145) image](/ps/datasheet/Images/3/ab3145/ab3145_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at 2 µg/mg of lysate. (with protein G). Native verified.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
Application notes
Is unsuitable for ,IHC-Fr or IHC-P.
Target
Relevance
Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Database links
- Entrez Gene: 7298 Human
- Omim: 188350 Human
- SwissProt: P04818 Human
- Unigene: 592338 Human
Anti-Thymidylate Synthase antibody [TS 106] images:
Western blot - Thymidylate Synthase antibody [TS 106] (ab3145)
Anti-Thymidylate Synthase antibody [TS 106] (ab3145) at 1 µg/ml + HL60 whole cell lysate at 20 µg
Secondary
HRP conjugated sheep F(ab') anti-mouse
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
References for Anti-Thymidylate Synthase antibody [TS 106] (ab3145)
This product has been referenced in:
- Novotny-Diermayr V et al. SB939, a novel potent and orally active histone deacetylase inhibitor with high tumor exposure and efficacy in mouse models of colorectal cancer. Mol Cancer Ther 9:642-52 (2010). WB; Human.Read more (PubMed: 20197387) »
- Perotti A et al. Phase IB study of the mTOR inhibitor ridaforolimus with capecitabine. J Clin Oncol 28:4554-61 (2010). WB; Human.Read more (PubMed: 20855840) »
See all 7 publications for this product
Publishing research using ab3145? Please let us know so that we can cite the reference in this datasheet
![Thymidylate Synthase antibody [TS 106] for WB in Human (3145)](/ps/Reviews/Images/ab7836_33349.jpg)
![Thymidylate Synthase antibody [TS 106] for WB in Human (3145)](/ps/Reviews/Images/ab13314_77989.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"