Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
Key features and details
- Rabbit polyclonal to Thyroid Hormone Receptor alpha
- Suitable for: WB, IHC-P, ChIP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Thyroid Hormone Receptor alpha antibody
See all Thyroid Hormone Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Thyroid Hormone Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Thyroid Hormone Receptor alpha aa 8-59.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (7) |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. -
Involvement in disease
Hypothyroidism, congenital, non-goitrous, 6 -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7067 Human
- Entrez Gene: 21833 Mouse
- Omim: 190120 Human
- SwissProt: P10827 Human
- SwissProt: P63058 Mouse
- Unigene: 724 Human
- Unigene: 265917 Mouse
- Unigene: 442648 Mouse
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Alternative names
- AR7 antibody
- c-erbA-1 antibody
- C-erbA-alpha antibody
see all
Images
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All lanes : Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/1000 dilution
Lane 1 : HeLa
Lane 2 : HepG2
Lane 3 : CACO2
Lane 4 : SW480
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaThe molecular weight observed is consistent with what has been described in the literature.
Lot: 811200345
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Chromatin was prepared from C2C12 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab53729 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
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ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab53729 has been referenced in 13 publications.
- Zhu X et al. 2,3',4,4',5-Pentachlorobiphenyl attenuated fast-twitch fibers and fiber size of skeletal muscle via disturbing thyroid hormone signaling and mitochondrial dynamics. J Appl Toxicol 42:1628-1638 (2022). PubMed: 35411558
- Major E et al. Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase. Int J Mol Sci 22:N/A (2021). PubMed: 34638630
- Ntari L et al. Changes in Thyroid Hormone Signaling Mediate Cardiac Dysfunction in the Tg197 Mouse Model of Arthritis: Potential Therapeutic Implications. J Clin Med 10:N/A (2021). PubMed: 34884213
- Olichwier A et al. Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart. Int J Mol Sci 22:N/A (2020). PubMed: 33374300
- Liang X et al. Thyroid function alterations attributed to high iodide supplementation in maternal rats and their offspring. J Trace Elem Med Biol 47:89-97 (2018). PubMed: 29544812