Tissue Factor Pathway Inhibitor Human ELISA Kit (ab108904)
Overview
- Product nameTissue Factor Pathway Inhibitor Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.3 ng/ml
- Range0.313 ng/ml - 20 ng/ml
- Recovery
98 %
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Tissue Factor Pathway Inhibitor (TFPI) is an endogenous protease inhibitor that regulates the initiation of the extrinsic coagulation pathway by producing factor Xa-mediated feedback inhibition of the tissue factor/factor VIIa (TF/FVIIa) catalytic complex. TFPI has a negatively charged amino terminus, three tandem Kunitz proteinase inhibitory domains, and a positively charged carboxy terminus. The first Kunitz domain is the binding site for the TF/FVIIa complex and the second domain for factor Xa. The resultant quaternary complex of TFPI/FXa/TF/FVIIa lacks TF/FVIIa catalytic activity. The third Kunitz-type domain and the carboxy-terminus of TFPI mediate its binding to heparin and cell surfaces including the endothelium. TFPI is synthesized mainly by endothelial cells and present in three pools in vivo: 10% in platelets, in endothelium associated with endothelial glyco-saminoglycans, and in plasma circulating as free or lipoprotein associated forms. The plasma TFPI contains mostly 34 and 40 kDa forms and the concentration is approximately 50 to 100 ng/ml. Measurement of TFPI could be important in thrombogenesis, atherosclerosis and heparinization studies. Higher plasma levels of TFPI were found in older individuals, pregnant women and patients with advanced cancer.
ab108904 Tissue Factor Pathway Inhibitor Human ELISA kit is designed for detection of Human TFPI in plasma, serum, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TFPI in less than 4 hours. A polyclonal antibody specific for TFPI has been pre-coated onto a 96-well microplate with removable strips. TFPI in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for TFPI, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated TFPI Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl TFPI Microplate 1 x 96 units TFPI Standard 1 unit Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
- SwissProt: P10646 Human
Applications
Our Abpromise guarantee covers the use of ab108904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Tissue Factor Pathway Inhibitor Human ELISA Kit images
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Sandwich ELISA - Tissue Factor Pathway Inhibitor Human ELISA Kit (ab108904)
Protocols
References for Tissue Factor Pathway Inhibitor Human ELISA Kit (ab108904)
ab108904 has not yet been referenced specifically in any publications.
