Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)
- Product nameTissue type Plasminogen Activator Human ELISA Kit (with color giving dyes)See all Tissue type Plasminogen Activator kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 3.6%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity6 pg/ml
- Range15.6 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 98 77% - 109%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
ab119563 Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Tissue type Plasminogen Activator. ab119563 is for research use only. Not for diagnostic or therapeutic procedures.
Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling.
Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 2 units Assay Buffer Concentrate (20x) 1 x 5ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml HRP-Conjugate anti-human Tissue type Plasminogen Activator polyclonal antibody 1 x 100µl Human Tissue type Plasminogen Activator Standard 2 vials Polyclonal anti-human Tissue type Plasminogen Activator-coated microwell plate 1 unit Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
modificationsThe single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line.
- AlteplasePlasminogen activator tissuePlasminogen activator tissue type
- PLATReteplaset-PAT-plasminogen activatorTissue-type plasminogen activator chain BtPATPA_HUMAN
Our Abpromise guarantee covers the use of ab119563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) images
Representative standard curve using ab119563.
References for Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)
ab119563 has not yet been referenced specifically in any publications.