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Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)

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Overview

  • Product nameTissue type Plasminogen Activator Human ELISA Kit (with color giving dyes)See all Tissue type Plasminogen Activator kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.6%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    6 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 98 77% - 109%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119563 Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of Human Tissue type Plasminogen Activator. ab119563 is for research use only. Not for diagnostic or therapeutic procedures.

    Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling.

    Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Adhesive Films 2 units
    Assay Buffer Concentrate (20x) 1 x 5ml
    Blue-Dye 1 x 0.4ml
    Green-Dye 1 x 0.4ml
    HRP-Conjugate anti-human Tissue type Plasminogen Activator polyclonal antibody 1 x 100µl
    Human Tissue type Plasminogen Activator Standard 2 vials
    Polyclonal anti-human Tissue type Plasminogen Activator-coated microwell plate 1 unit
    Stop Solution 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    Substrate Solution 1 x 15ml
    Wash Buffer Concentrate (20x) 1 x 50ml
  • Research Areas
  • FunctionConverts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • Tissue specificitySynthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • Involvement in diseaseNote=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • DomainBoth FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • Post-translational
    modifications    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • Cellular localizationSecreted > extracellular space.

    • Target information above from: UniProt accession P00750 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        AlteplasePlasminogen activator tissuePlasminogen activator tissue type
        PLATReteplaset-PAT-plasminogen activatorTissue-type plasminogen activator chain BtPATPA_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119563 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)
        Sandwich ELISA - Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)
        Representative standard curve using ab119563.

      Protocols

      References for Tissue type Plasminogen Activator Human ELISA Kit (with color giving dyes) (ab119563)

      ab119563 has not yet been referenced specifically in any publications.

      Publishing research using ab119563 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"