Anti-Titin antibody [T11] (ab7034)

I'm interested in your anti-Titin. You can use this antibody for western blot but I don't have a protocol for a protein that big.The standard protocols are not designed for such high weight molecules. Do you have a protocol for this? If you have can you send it to me. Thanks

ANSWER:

Thank you for your enquiry regarding separation on WB of large proteins.

I would recommend using a 5% 1cm thick gel and to change the ratio of bis-acrylamide to mono-acrylamide from the classical 37:1 ratio to a 49:1 ratio.

For the transfer I recommend adding a little SDS to the transfer buffer to help.

Please let me know if you need further information,

Please, send me protocol of titin extraction and purification, materials

ANSWER:

Thank you for your enquiry.

Development of the antigen for antibody production is a confidential/proprietary process which we do not have authority to detail at this time.

However, you may find some useful information at the following websites:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=6512859&dopt=Abstract

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=2117014

By DD-PCR,we found that titin can be induced to express on trophoblasts.We blasted the DD-PCR product(92 bp) to geneBank,they are the same as the 404th to 495th bases of novex mRNA.now I want to detect the DD-PCR products in protein level Can I use the antibody of titin(ab7034)? I hope Ican get your kindly help soon.Thanks a lot.

ANSWER:

ab7034 has not been used in this way and therefore we have no further information on whether it will be suitable for your application. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Dear Sir:

I am interested in your product Titin antibody (ab7034) and have some questions about this antibody. 1) Can we use this antibody to do western blot for human tissue sample? What molecular weight Titin can be detected? 2) Do you have or suggest protocol of tissue sample preparation for this western blot? 3) Do you have this antibody dada sheet of western blot? If you have, could you please send it to me? Thanks

Anguo Gu MD Division of Circulatory Physiology Columbia University

ANSWER:

Yes, you can use this antibody for Western blotting on human tissue samples.

I am afraid that we don't know the size that Titin runs on a Western blot. I do know that the antibody detects a doublet though......we don't have a Western blot picture using this antibody.

As we rely on feedback from customers and other sources for a lot of our data, we sometimes don't get all the information we want, but we feel that limited information is better than none. We would rather present you with all the information we have and let the customer decide.

I don't have a specific protocol for lysate preparation for use with this antibody, I can only suggest you check the literature. I know that as titin has a very high molecular weight you will find it difficult to use standard protocols. I did see an interesting looking paper on this recently (Electrophoresis. 2003 Jun;24(11):1695-702.) I believe you should find some useful advice in there.

I hope this helps.

I have few questions about the titin - ab7034 antibody, you carry. I would like to get your conformation that is reacts with the human cardiac tissue! Do you know the epitope of the antibody? Approximately. Is it C-termin. or N-termin? For Western blotting what dilution is needed? Would you be so kind to make a bid for the above mentioned product?

ANSWER:

Yes, this antibody should cross react with human cardiac muscle. We have no information on the location of the epitope. The concentration of the antibody is 5.10 mg/ml - I'd start with a 1:2000 dilution. Price information in GBP/ USD/ EUR can be found by clicking on the prices icon on the datasheet