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- Secondary antibodies
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Product name
Anti-Tollip antibody
See all Tollip products (7) ...
Description
Rabbit polyclonal to Tollip
Tested applications
IHC-P, ELISA, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.
Positive control
Rat brain cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Immunology >> Immunoglobulins >> Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC: Use a concentration of 2 µg/ml.
Target
Function
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
Sequence similarities
Belongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain.
Post-translational
modifications
Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9H0E2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
see all
Database links
- Entrez Gene: 54472 Human
- Entrez Gene: 54473 Mouse
- Entrez Gene: 361677 Rat
- Omim: 606277 Human
- SwissProt: Q9H0E2 Human
- SwissProt: Q9QZ06 Mouse
- SwissProt: A2RUW1 Rat
- Unigene: 368527 Human
see all
Anti-Tollip antibody images:
Western blot - Tollip antibody (ab37155)
Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml
Lane 1 : Rat brain cell lysate
Lane 2 : Rat brain cell lysate
Predicted band size : 30 kDa
Observed band size : 30 kDa
Immunocytochemistry - Tollip antibody (ab37155)
ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Tollip antibody(ab37155)
ab37155 staining Tollip in human placenta.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Tollip antibody (ab37155)
ab37155 has not yet been referenced specifically in any publications.
Publishing research using ab37155? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"