Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
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ab3825 has been referenced in 6 publications.
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blue: DAPI; green: Topo I. Hela cell staining.
Predicted band size : 91 kDa
Primary antibody incubated at a 1/1000 dilution for 60 minutes at 25ºC.
All lanes : Anti-Topoisomerase I antibody - ChIP Grade (ab3825) at 1/5000 dilution
Lane 1 : SW210.5 human SCLC cell xenograft lysate.
Lane 2 : 5M2 human NSCLC cell lysate (bicarb. buffer).
Lane 3 : 5M2 human NSCLC cell lysate (M-Per buffer).
Lane 4 : Normal human lung tissue lysate.
Lane 5 : A431 cell (human epidermoid carcinoma) lysate .
Lane 6 : Rh30 cell (human rhabdomyosarcoma) lysate.
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti rabbit heavy and light (HRP) at 1/25000 dilution
Performed under reducing conditions.
Predicted band size : 91 kDa
This image is courtesy of an Abreview submitted by Mike Campa on 7 September 2005. We do not have any further information relating to this image.
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