Anti-Topoisomerase I antibody - ChIP Grade (ab3825)

Hi, I was wondering if this antibody would work with Arabidopsis top1a.

ANSWER:

Thank you for contacting us.

To our knowledge, reactivity of the antibody ab3825 with Arabidopsis Top1a has never been tested.

To check the probability of cross-reaction, I aligned the amino acid sequence of Arabidopsis Top1a (found at this link: http://www.uniprot.org/uniprot/P30181) with the sequence of the immunogen of the antibody, which is the fragment from amino acids 401 - 600 of human Top1a (found at this link: http://www.uniprot.org/uniprot/P11387)

The result is that there is only 53% overall identity, but also some regions of 10- 20 amino acids that are completely conserved.

In short, the antibody may recognize Arabidopsis Top1a, if it recognizes one or more of the regions that are conserved. The antibody is polyclonal, that is, it recognizes multiple epitopes, but the locations of the epitopes are unknown.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I have already submitted the abreview for the results of testing ab3825 in a mouse cell line. However, I was not able to find the 'additional comments' section to put in my discount code. Can you please let me know whether or not my discount code can be approved? Thank you very much!

ANSWER:

Thank you for your email.

I've added the discount code to your review, so it is now active and can be redeemed for a free primary antibody. Thank you for your review!

Please let me know if you need anything else. Have a great day!

Would like to try this antibody with mouse samples.

ANSWER:

Thank you for your call today. I've issued you the discount code below, and let me know if you have any questions or if there is anything else that we can do for you.

DISCOUNT CODE: ****
Expiration date: August 11, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab3825 in mouse samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

BATCH NUMBER 87273 ORDER NUMBER 71086

DESCRIPTION OF THE PROBLEM I just took out the vial from the refridgerater where the product has been stored since it was shipped to me along side the other antibody that I purchased. Unfortunately, the product is all dried up at the bottom of the vial. THe cap is on tightly and there are no cracks in the vial that I can detect. Would you please investigate this matter for me and, if possible, consider replacing it? Thank you.

ANSWER:

Thank you for your enquiry. The ammonium sulfate-precipitated anti-topoisomerase I antiserum is kept in PBS with 1% BSA and then lyophilized. Lyophilized ab3825 antibody should be reconstituted with 100 ul of pure H2O before use.

Please let me know if you need further assistance.

i am having problems getting a topo1 antibody to work with immuno fluorescence. I am just about to order your antibody and would like all the info you have on immunofluorescence of cells (not sections) ie fixation, ab concs and incubations etc

ANSWER:

Thank you for your enquiry. All the information that we have regarding ab3825 and its application in immunofluorescence is located on the online datasheet. If you have any more questions, please contact us again.