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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Overview
Product name
Anti-Topoisomerase I antibody [Mab1]
See all Topoisomerase I products (7) ...
Description
Mouse monoclonal [Mab1] to Topoisomerase I
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human).
Positive control
Extract of A231 human kidney cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
Mab1
Myeloma
Sp2/0
Isotype
IgG1
Research areas
Cancer >> Drug resistance >> Topoisomerases
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
Target
Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Involvement in disease
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Sequence similarities
Belongs to the eukaryotic type I topoisomerase family.
Post-translational
modifications
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
Target information above from: UniProt accessionP11387
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
Database links
- Entrez Gene: 7150 Human
- Omim: 126420 Human
- SwissProt: P11387 Human
- Unigene: 472737 Human
- Unigene: 712686 Human
References for Anti-Topoisomerase I antibody [Mab1] (ab12407)
ab12407 has not yet been referenced specifically in any publications.
Publishing research using ab12407? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"