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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Overview
Product name
Anti-Topoisomerase I antibody
See all Topoisomerase I products (7) ...
Description
Rabbit polyclonal to Topoisomerase I
Specificity
Ab28432 recognises Topoisomerase I.
Tested applications
WB, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length Topoisomerase I protein (Human).
Positive control
HeLa nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Ab28432 is affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Drug resistance >> Topoisomerases
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 2 Images
ICC/IF
ELISA
Application notes
ELISA: 1/500 (PMID 19584232).
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/2000 - 1/5000. Predicted molecular weight: 91 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Involvement in disease
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Sequence similarities
Belongs to the eukaryotic type I topoisomerase family.
Post-translational
modifications
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
Target information above from: UniProt accessionP11387
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
Database links
- Entrez Gene: 7150 Human
- Omim: 126420 Human
- SwissProt: P11387 Human
- Unigene: 472737 Human
- Unigene: 712686 Human
Anti-Topoisomerase I antibody images:
Immunocytochemistry/ Immunofluorescence-Topoisomerase I antibody(ab28432)
ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Topoisomerase I antibody (ab28432)
ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Topoisomerase I antibody (ab28432)
This product has been referenced in:
- D'Onofrio Get al. Poly(ADP-ribose) polymerase signaling of topoisomerase 1-dependent DNA damage in carcinoma cells. Biochem Pharmacol 81:194-202 (2011). WB; Human.Read more (PubMed: 20875401) »
- Pfister TDet al. Topoisomerase I levels in the NCI-60 cancer cell line panel determined by validated ELISA and microarray analysis and correlation with indenoisoquinoline sensitivity. Mol Cancer Ther 8:1878-84 (2009). ELISA; Human.Read more (PubMed: 19584232) »
See all 3 publications for this product
Publishing research using ab28432? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"