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Immunocytochemistry/ Immunofluorescence-Topoisomerase I antibody(ab28432)
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Product Name
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Topoisomerase I antibody
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See all Topoisomerase I antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Topoisomerase I
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Immunogen
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Recombinant full length Topoisomerase I protein (Human).
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Reacts with
(species key)
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Hu
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Specificity
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Ab28432 recognises Topoisomerase I.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/500 (PMID 19584232).
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/2000 - 1/5000. Predicted molecular weight: 91 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa nuclear extract.
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Cellular localization
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Cytoplasmic and Nuclear.
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Research areas
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Cancer >> Drug resistance >> Topoisomerases
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
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Relevance
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Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Purification notes
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Ab28432 is affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Topoisomerase I antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Topoisomerase I antibody images, references, products related to ab28432 and other tools.
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Topoisomerase I antibody images:
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 Immunocytochemistry/ Immunofluorescence-Topoisomerase I antibody(ab28432)
ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Topoisomerase I antibody (ab28432)
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This product has been used in: (two most recent references)
Pfister TD et al. Topoisomerase I levels in the NCI-60 cancer cell line panel determined by validated ELISA and microarray analysis and correlation with indenoisoquinoline sensitivity. Mol Cancer Ther 8:1878-84 (2009). ELISA; Human. PubMed: 19584232
Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB; Human. PubMed: 19023046
If you publish research using ab28432 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Topoisomerase I
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Topoisomerase I antibody - more information
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Customer reviews (feedback) regarding Topoisomerase I antibody |
Customer FAQs regarding Topoisomerase I antibody |
Protocols for Topoisomerase I antibody |
Price and availability of products related to Topoisomerase I antibody |
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Topoisomerase I antibody - other tools:
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Contact Abcam with a Technical Enquiry about Topoisomerase I antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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