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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Overview
Product name
Anti-Topoisomerase I antibody
See all Topoisomerase I products (7) ...
Description
Mouse monoclonal to Topoisomerase I
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF, corresponding to amino acids 692-766 of Human Topoisomerase I
QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Drug resistance >> Topoisomerases
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 1-5 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Involvement in disease
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Sequence similarities
Belongs to the eukaryotic type I topoisomerase family.
Post-translational
modifications
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
Target information above from: UniProt accessionP11387
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
Database links
- Entrez Gene: 7150 Human
- Omim: 126420 Human
- SwissProt: P11387 Human
- Unigene: 472737 Human
- Unigene: 712686 Human
Anti-Topoisomerase I antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Topoisomerase I antibody (ab58313)
Topoisomerase I antibody (ab58313) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma.
Western blot - Topoisomerase I antibody (ab58313)
Western blot against tagged recombinant protein immunogen using ab58313 Topoisomerase I antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
References for Anti-Topoisomerase I antibody (ab58313)
ab58313 has not yet been referenced specifically in any publications.
Publishing research using ab58313? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"