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Western blot - Torsin A antibody (ab34540)
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Western blot - Torsin A antibody (ab34540)
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Immunocytochemistry/ Immunofluorescence - Torsin A antibody (ab34540)
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Product Name
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Torsin A antibody
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See all Torsin A antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Torsin A
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Rat Torsin A. (Peptide available as ab35510.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the following mouse lysates:
Brain tissue
Testis tissue
Heart tissue
Skeletal Muscle tissue
Liver tissue
Mouse Fetal lysate - 14 Days old Tissue
Brain Tissue Lysate - normal tissue, 0 days old
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell
MEF1 (Mouse embryonic fibroblast cell line) Whole Cell
This antibody gave a positive control in the following rat lysates:
Liver tissue
PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell
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Cellular localization
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Endoplasmic reticulum
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Research areas
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Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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Mutations in Torsin A (Dyt1 gene) causes early onset torsin dystonia, a movement disorder involving dysfunction in the basal ganglia without apparent neurodegeneration. Most cases result from a dominantly acting three-base pair deletion in the Dyt1 gene causing loss of a glutamic acid near the carboxyl terminus of Torsin A. Torsins are members of the AAA(+) superfamily of ATPases and are present in all multicellular organisms. Torsin A is an ER protein involved in chaperone functions and/or membrane movement. Torsins are also implicated in neurogenesis, neuronal migration, axon/dendrite development and synaptogenesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Torsin A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Torsin A antibody images, references, products related to ab34540 and other tools.
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Torsin A antibody images:
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 Western blot - Torsin A antibody (ab34540)
All lanes : Torsin A antibody (ab34540) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 38 kDa
Observed band size : 38 kDa
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Torsin A antibody (ab34540)
ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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References for Torsin A antibody (ab34540)
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This product has been used in:
Granata A et al. The Dystonia-associated Protein TorsinA Modulates Synaptic Vesicle Recycling. J Biol Chem 283:7568-79 (2008). ICC/IF; Human. PubMed: 18167355
If you publish research using ab34540 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Torsin A
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Torsin A antibody - more information
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Customer reviews (feedback) regarding Torsin A antibody |
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