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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-Torsin A antibody
See all Torsin A products (3) ...
Description
Rabbit polyclonal to Torsin A
Tested applications
IHC-FoFr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Rat Torsin A.
(Peptide available as ab35510.)
Positive control
This antibody gave a positive control in the following mouse lysates: Brain tissue Testis tissue Heart tissue Skeletal Muscle tissue Liver tissue Mouse Fetal lysate - 14 Days old Tissue Brain Tissue Lysate - normal tissue, 0 days old NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell MEF1 (Mouse embryonic fibroblast cell line) Whole Cell This antibody gave a positive control in the following rat lysates: Liver tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: 1/100
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Target
Relevance
Mutations in Torsin A (Dyt1 gene) causes early onset torsin dystonia, a movement disorder involving dysfunction in the basal ganglia without apparent neurodegeneration. Most cases result from a dominantly acting three-base pair deletion in the Dyt1 gene causing loss of a glutamic acid near the carboxyl terminus of Torsin A. Torsins are members of the AAA(+) superfamily of ATPases and are present in all multicellular organisms. Torsin A is an ER protein involved in chaperone functions and/or membrane movement. Torsins are also implicated in neurogenesis, neuronal migration, axon/dendrite development and synaptogenesis.
Cellular localization
Endoplasmic reticulum
Alternative names
- DQ2 antibody
- Dystonia 1 antibody
- Dystonia 1 protein antibody
see all
Database links
- Entrez Gene: 1861 Human
- Entrez Gene: 30931 Mouse
- Entrez Gene: 266606 Rat
- Omim: 605204 Human
- SwissProt: O14656 Human
- SwissProt: Q9ER39 Mouse
- SwissProt: Q68G38 Rat
- SwissProt: Q8K3L8 Rat
Anti-Torsin A antibody images:
Western blot - Torsin A antibody (ab34540)
All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Mouse Brain at 10 µg
Lane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
Lane 4 : Mouse Heart Mouse Tissue Lysate at 10 µg
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711) at 10 µg
Lane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
Lane 7 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µg
Lane 8 : Mouse Embryonic Fibroblast 1 at 10 µg
Lane 9 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 38 kDa
Observed band size : 38 kDa
Additional bands at : 105 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Western blot - Torsin A antibody (ab34540)
All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 38 kDa
Observed band size : 38 kDa
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Torsin A antibody (ab34540)
ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (PFA perfusion fixed frozen sections) - Torsin A antibody (ab34540)
IHC-FoFr image of Torsin A (ab34540) on Rat Cerebral Cortex section. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Sophie Pezet, ESPCI, France
References for Anti-Torsin A antibody (ab34540)
This product has been referenced in:
- Sciamanna Get al. Developmental profile of the aberrant dopamine D2 receptor response in striatal cholinergic interneurons in DYT1 dystonia. PLoS One 6:e24261 (2011). IHC-P; Mouse.Read more (PubMed: 21912682) »
- Kim CEet al. A molecular mechanism underlying the neural-specific defect in torsinA mutant mice. Proc Natl Acad Sci U S A 107:9861-6 (2010). WB, ICC/IF; Mouse.Read more (PubMed: 20457914) »
See all 6 publications for this product
Publishing research using ab34540? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"