Overview

  • Product nameTotal Antioxidant Capacity Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Assay time
    2h 00m
  • Product overview

    Abcam's Total Antioxidant Capacity Assay Kit can measure either the combination of both small molecule antioxidants and proteins or small molecules alone in the presence of our proprietary Protein Mask. Cu2+ ion is converted to Cu+ by both small molecule and protein. The Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Antioxidants play an important role in preventing the formation of and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Different antioxidants vary in their reducing power. Trolox is used to standardize antioxidants, with all other antioxidants being measured in Trolox equivalents. Measurement of the combined non-enzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases. In some cases, the antioxidant contribution of proteins is desired whereas in other cases only the contribution of the small molecule antioxidants is needed.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceAntioxidants play an important role in preventing the formation of and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Different antioxidants vary in their reducing power, and cooperation of all different antioxidants provides greater protection against reactive oxigen or nitrogen radicals than any single compound alone. Therefore, the overall total antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present.

Applications

Our Abpromise guarantee covers the use of ab65329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Total Antioxidant Capacity Assay Kit images

Protocols

References for Total Antioxidant Capacity Assay Kit (ab65329)

This product has been referenced in:
  • Csillag A  et al. Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model. J R Soc Interface 11:20140097 (2014). Read more (PubMed: 24647908) »
  • Enseleit F  et al. Effects of Pycnogenol on endothelial function in patients with stable coronary artery disease: a double-blind, randomized, placebo-controlled, cross-over study. Eur Heart J : (2012). Functional Studies . Read more (PubMed: 22240497) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry.

Generally, serum can be stored at -20°C or at -70°. Freeze the tubes of serum rapidly, and store the aliquots protected from light. After thawing a tube, do not refreeze the serum unless you intend to store...

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Yes, the kit can be used with samples from chicken tissues. We have not generated or received chicken data but the assay is suitable for any species. For an example of data from mouse heart tissue, please see figure 4 of the following reference:
...

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Unopened kit as received from Abcam can be stored for 6 months at 4C. Once reconstituted the standard should be stored at -20C. Please refer to the protocol booklet for further information.

If the total antioxidant capacity is desired, I would not recommend the use of the protein mask. If only the levels of the small molecule antioxidants are required, please use the protein mask.

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Although I don’t think EDTA will have an adverse effect on the efficiency of this kit, I would not recommend using EDTA for plasma preparations. Instead use heparin or citrate.

EDTA anticoagulant w...

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This kit has only been optimized for mammalian sample. Theoretically, it could be used in samples of multiple species including plant. Because we have not tested it with any plant sample, I am sorry that there is no specific protocol for measuring plan...

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I am sorry to confirm we have not specifically tested mouse spermatozoa using this kit. But since the kit is compatible with cells of murine origin, I do not see why it would not work.

We would rec...

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Thank you for your enquiry regarding ab65329.

As the datasheet indicates different biological fluids can be used with this assay kit. Although we have not used ab65329 with saliva samples - this type of same would be compatible. I would recom...

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With regards to your questions:

1. DMSO is a solvent that dissolves both polar and nonpolar compounds. In this case DMSO is used to dissolve lyophilized Trolox standard.

2. The type of standar...

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Thank you for contacting us. Yes, this kit will be suitable for use on rat liver samples.

Here is the protocol for tissue homogenization for this kit: Start with 10-100 mg of the tissue, add 500-1000 ul (or ˜4-6 volumes) of PBS on ice, homoge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"