Overview
- Product nameAnti-GNB1 antibodySee all GNB1 primary antibodies ...
- DescriptionRabbit polyclonal to GNB1
- Tested applicationsWB more details
- Species reactivityReacts with: Sheep, Human
Predicted to work with: Rat, Cow, Amphibians - Immunogen
Synthetic Peptide: R(8)QEAEQLKNQIRDARKAC(25). Immunizing peptide corresponds to amino acid residues 8-25 from human GNB1. This sequence is completely conserved between human, amphibian, bovine and rat.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS, 1 mg/ml BSA and 0.05% sodium azide
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Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notes Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to, and interpreted by, the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab3433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB |
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects an ~37 kDa protein representing Transducin beta from sheep retinal extract.
Target
- FunctionGuanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
- Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats. - Post-translational
modificationsPhosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. -
Database links
- Entrez Gene: 281201 Cow
- Entrez Gene: 2782 Human
- Entrez Gene: 24400 Rat
- Omim: 139380 Human
- SwissProt: P62871 Cow
- SwissProt: P62873 Human
- SwissProt: P54311 Rat
- Unigene: 430425 Human
- Unigene: 126047 Rat
see all
Target information above from: UniProt accession
P62873
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Beta subunit signal transducing proteins GS/GI antibodyG protein beta 1 subunit antibodyGBB1 antibody
- GBB1_HUMAN antibodygnb1 antibodyGuanine nucleotide binding protein (G protein) beta polypeptide 1 antibodyGuanine nucleotide binding protein beta 1 subunit antibodyGuanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 antibodyTransducin beta chain 1 antibody
see all
Anti-GNB1 antibody images
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Western blot - GNB1 antibody (ab3433)Western blot of GNB1 on sheep retinal extracts using ab3433.
References for Anti-GNB1 antibody (ab3433)
ab3433 has not yet been referenced specifically in any publications.
