Overview
- Product nameTransferrin Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich
- Sensitivity= 1.5 ng/ml
- Range1.563 ng/ml - 100 ng/ml
- Recovery
96 %
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Transferrin is a plasma protein that transports iron through the blood to the liver, spleen and bone marrow. Low Transferrin level in plasma could associate with anemia, and chronic liver disease. On the other hand, high plasma Transferrin level could indicate iron deficiency anemia .
ab108902 Transferrin Human ELISA kit is designed for detection of Human Transferrin in urine, milk, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Transferrin in less than 4 hours. A polyclonal antibody specific for Transferrin has been pre-coated onto a microplate. Transferrin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Transferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Transferrin Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Transferrin Microplate (12 strips of 8 wells) 1 unit Human Transferrin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
- SwissProt: P02787 Human
Applications
Our Abpromise guarantee covers the use of ab108902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Transferrin Human ELISA Kit images
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ELISA - Transferrin Human ELISA KitELISA analysis of Transferrin in HepG2 cell conditioned media and fresh growth media containing FBS, using ab108902 Transferrin Human ELISA Kit. This image is courtesy of an anonymous Abreview. -
Sandwich ELISA - Transferrin Human ELISA Kit - 1 x 96 well plate (ab108902)
Protocols
References for Transferrin Human ELISA Kit (ab108902)
ab108902 has not yet been referenced specifically in any publications.
