Transferrin Human ELISA Kit (ab108902)

Overview

  • Product nameTransferrin Human ELISA Kit
    See all Transferrin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.563 ng/ml - 100 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.

    A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseAtransferrinemia
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • ATRAF
    • Atransferrinemia
    • Beta-1 metal-binding globulin
    • PRO1400
    • Serotransferrin
    • Siderophilin
    • TF
    • Transferrin
    • TRFE_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Transferrin Human ELISA Kit images

  • Representative Standard Curve using ab108902.

Protocols

References for Transferrin Human ELISA Kit (ab108902)

ab108902 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in awaiting our reply in the matter of compaibility with the kit. It is difficulty to know whether this will work. If the conjugation block the antibody binding site, it will interfere the assay. If not, it will be fine. ...

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Abreview for Transferrin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.1ug standard µg
Type of Kit Used: ELISA
Positive control: Provided transferrin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human transferrin. Did not recognize bovine transferrin in FBS.
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Abcam user community

Verified customer

Submitted Jul 04 2012

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"