![Western blot - Transferrin Receptor antibody [B349 (DF1513)] (ab8598) image](#ab8598_1.jpg)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor antibody [B349 (DF1513)]
See all Transferrin Receptor products (58) ...
Description
Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
Specificity
This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.
Tested applications
WB, Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Cell line KG1.
Positive control
Tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
Clonality
Monoclonal
Clone number
B349 (DF1513)
Myeloma
unknown
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
![Western blot - Transferrin Receptor antibody [B349 (DF1513)] (ab8598) image](/ps/datasheet/Images/8/ab8598/ab8598_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
Anti-Transferrin Receptor antibody [B349 (DF1513)] images:
Western blot - Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
All lanes : Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) at 1/100 dilution
Lane 1 : Human U2OS cell line, whole cell lysate
Lane 2 : Human 293T cell line, whole cell lysate
Lane 3 : African Green Monkey Cos7 cell line, whole cell lysate
Lane 4 : Hamster CHO cell line, whole cell lysate
Lane 5 : Rat PC12 cell line, whole cell lysate
Lane 6 : Mouse 3T3 cell line, whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 7 minutes
This image is courtesy of an anonymous Abreview
References for Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598)
This product has been referenced in:
- Ortiz-Zapater Eet al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006).Read more (PubMed: 17059402) »
- Ayala Pet al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005).Read more (PubMed: 16309460) »
See all 2 publications for this product
Publishing research using ab8598? Please let us know so that we can cite the reference in this datasheet
![Transferrin Receptor antibody [B349 (DF1513)] for WB in Human (8598)](/ps/Reviews/Images/ab8939_43123.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"