Transferrin Receptor antibody [B349 (DF1513)] (ab8598)

Product Name 

Transferrin Receptor antibody [B349 (DF1513)]

Product type 

Primary antibodies

Description 

Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor

Immunogen 

Cell line KG1.

Reacts with 
(species key)

Hu

Specificity 

This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond.
The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9.
This antibody reacts with many proliferating cells in both normal and neoplastic tissues.
It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/100.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil.

Cellular localization 

Type II membrane protein. Secreted (isoform sTfR).

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

This antibody is an indicator of proliferation activity.
It also has prognostic significance when typing tumors, such as leukemias and lymphomas.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B349 (DF1513)

Isotype 

IgG1

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

50 mM borate, 150 mM NaCl, 20% glycerol and 0.05% sodium azide, pH 8.0.

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for Transferrin Receptor antibody [B349 (DF1513)] images, references, products related to ab8598 and other tools.

Transferrin Receptor antibody [B349 (DF1513)] images:

References for Transferrin Receptor antibody [B349 (DF1513)] (ab8598)

  This product has been used in: (two most recent references)

Ortiz-Zapater E et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). PubMed: 17059402

Ayala P et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). PubMed: 16309460
If you publish research using ab8598 please let us know so that we can cite the reference on this datasheet.

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