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Hi, I'm looking for immunostain fozen section of human cell xenograft in mouse transferrin receptor. I wonder why these two antibodies all clone DF1513, are they the same? Which ab is good for IHC frozen? any reference?
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ANSWER: |
Thank you for your enquiry. Ab8598 and Ab7350 are the same clone, DF1513. We get these two antibodies from two different sources. Ab8598 has been tested for application in IHC-frozen sections. Unfortunately, we are not aware of any publications that feature the use of this antibody. |
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Customer would like to know...what is a good positive control for ab7350? She is using it in FACS. |
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ANSWER: |
Thank you for your enquiry. Any human cell line eg. KG1, U266, MOLT4 (i.e. anything that is proliferating well) would represent a suitable positive control for this antibody in flow cytometry.
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Could I use this antibody on monolayer cells only fixed with 4% formaldehyde/PBS? ie. there is no paraffin embedding etc. Thanks Dr. C. Berry |
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ANSWER: |
As we would not have tested the anti-human CD71 antibody in cell monolayers/smears we would be unable to guarantee its suitability for this procedure. However, having said that, fixing cells on slides and staining them is probably the most simple immunostaining method that does not have the drawbacks of antigen masking/damage common with other methods. There is no reason why it should not work and I can also confirm that 4% formaldehyde is a fixative commonly used in flow and therefore should not cause any problems. |
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