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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor antibody [DF1513] (Biotin)
See all Transferrin Receptor products (58) ...
Description
Mouse monoclonal [DF1513] to Transferrin Receptor (Biotin)
Conjugation
Biotin
Conjugation notes
Biotin
Specificity
DF1513 recognises the human CD71 cell surface antigen, a 190 kD homodimeric glycoprotein.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human, Rhesus monkey
Immunogen
KG1 acute myeloid leukemia cell line
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.2% BSA, 0.1% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.
Clonality
Monoclonal
Clone number
DF1513
Myeloma
NS1
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
Application notes
Flow cytometry: neat. Use 10 µl to label 106 cells.
Does not work in immunohistology on frozen and paraffin-embedded sections.
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
References for Anti-Transferrin Receptor antibody [DF1513] (Biotin) (ab7350)
This product has been referenced in:
- Ayala Pet al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005).Read more (PubMed: 16309460) »
See 1 publication for this product
Publishing research using ab7350? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"