Transferrin Receptor antibody [MEM-189] (ab1086)

Product Name 

Transferrin Receptor antibody [MEM-189]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MEM-189] to Transferrin Receptor

Immunogen 

Tissue / cell preparation (Human). (KG1 acute myelogenous leukaemia cell line).

Reacts with 
(species key)

Hu, Rat

Specificity 

Human CD71 (transferrin receptor). Does not block the binding of transferrin to it's receptor.

Tested applications 
(see key)

Flow Cyt, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependant concentration.
IP: Use at an assay dependant concentration.
WB: 1/1000 (see Abreview). Use under non-reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type II membrane protein. Secreted (isoform sTfR).

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MEM-189

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites by protein A-affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Transferrin Receptor antibody [MEM-189] (ab1086)

  This product has been used in: (two most recent references)

Xiang J et al. Mesenchymal stem cells as a gene therapy carrier for treatment of fibrosarcoma. Cytotherapy 11:516-26 (2009). ICC/IF; Rat. PubMed: 19562576

Barral DC et al. CD1a and MHC class I follow a similar endocytic recycling pathway. Traffic 9:1446-57 (2008). IP; Human. PubMed: 18564371

See all 4 publication references for this product.


If you publish research using ab1086 please let us know so that we can cite the reference on this datasheet.

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