![Immunocytochemistry/ Immunofluorescence - Transferrin Receptor antibody [MEM-189] (ab1086) image](#Transferrin-Receptor-Primary-antibodies-ab1086-3.jpg)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor antibody [MEM-189]
See all Transferrin Receptor products (58) ...
Description
Mouse monoclonal [MEM-189] to Transferrin Receptor
Specificity
The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
Tested applications
IHC-FrFl, Flow Cyt, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Tissue / cell preparation (Human). (KG1 acute myelogenous leukaemia cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by protein A-affinity chromatography.
Clonality
Monoclonal
Clone number
MEM-189
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
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Immunocytochemistry/ Immunofluorescence - Transferrin Receptor antibody [MEM-189] (ab1086)
![Immunocytochemistry/ Immunofluorescence - Transferrin Receptor antibody [MEM-189] (ab1086) image](/ps/datasheet/images/1/ab1086/Transferrin-Receptor-Primary-antibodies-ab1086-3.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab1086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FrFl: Use at an assay dependent concentration.
Flow Cyt: Use a concentration of 2 µg/ml
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration. Use under non reducing condition.
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Entrez Gene: 64678 Rat
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- SwissProt: Q99376 Rat
- Unigene: 529618 Human
see all
Anti-Transferrin Receptor antibody [MEM-189] images:
Immunocytochemistry/ Immunofluorescence - Transferrin Receptor antibody [MEM-189] (ab1086)
ab1086 at 1/100 dilution, staining Transferrin Receptor in rat mesenchymal stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde for 10 min at room temperature, washed with PBS (pH 7.4) and incubated with 10% serum plus background reducing components, for 30 min at room temperature. A Fluorescene conjugated anti-mouse IgG was used as secondry antibody and observed under an epi-fluorescence microscope (Nikon, Tokyo, Japan).
Image from Xiang J., et. al., Cytotherapy (2009) Vol. 11, No. 5, 516–526. (Fig 1C)
References for Anti-Transferrin Receptor antibody [MEM-189] (ab1086)
This product has been referenced in:
- Groppelli Eet al. Cell entry of the aphthovirus equine rhinitis A virus is dependent on endosome acidification. J Virol 84:6235-40 (2010).Read more (PubMed: 20375159) »
- Ornatsky Oet al. Highly multiparametric analysis by mass cytometry. J Immunol Methods 361:1-20 (2010). Flow Cyt; Human.Read more (PubMed: 20655312) »
See all 6 publications for this product
Publishing research using ab1086? Please let us know so that we can cite the reference in this datasheet
![Anti-Transferrin Receptor antibody [MEM-189] for Immunohistochemistry - Free Floating in Mouse (1086)](/ps/datasheet/images/1/ab1086/Transferrin-Receptor-Primary-antibodies-ab1086-12.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"