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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor antibody
See all Transferrin Receptor products (58) ...
Description
Chicken polyclonal to Transferrin Receptor
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
The immunogen corresponds to amino acids 89-298 of Human Transferrin Receptor.
Positive control
CD71 fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Transferrin Receptor antibody (ab37632)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
Anti-Transferrin Receptor antibody images:
Western blot - Transferrin Receptor antibody (ab37632)
Anti-Transferrin Receptor antibody (ab37632) at 1/2000 dilution + CD71 fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 85 kDa
Observed band size : 85 kDa
The arrow next to the image points to the Transferrin Receptor (CD71) fusion protein.
References for Anti-Transferrin Receptor antibody (ab37632)
ab37632 has not yet been referenced specifically in any publications.
Publishing research using ab37632? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"