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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor antibody
See all Transferrin Receptor products (58) ...
Description
Rabbit polyclonal to Transferrin Receptor
Specificity
Detects endogenous levels of total TfR protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic non phosphopeptide derived from human TfR around the phosphorylation site of serine 24 (RFSPLA).
Positive control
Extracts from 293 cells treated with PMA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Transferrin Receptor antibody (ab61134)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- Unigene: 529618 Human
- Unigene: 28683 Mouse
Anti-Transferrin Receptor antibody images:
Western blot - Transferrin Receptor antibody (ab61134)
All lanes : Anti-Transferrin Receptor antibody (ab61134) at 1/500 dilution
Lane 1 : extracts from 293 cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : extracts from 293 cells treated
with PMA (125ng/ml, 30mins) with immunizing peptide
Predicted band size : 85 kDa
Observed band size : 85 kDa
References for Anti-Transferrin Receptor antibody (ab61134)
This product has been referenced in:
- Tong Met al. Role of Key Aromatic Residues in the Ligand-binding Domain of {alpha}7 Nicotinic Receptors in the Agonist Action of {beta}-Amyloid. J Biol Chem 286:34373-81 (2011). WB.Read more (PubMed: 21828053) »
See 1 publication for this product
Publishing research using ab61134? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"