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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Transferrin Receptor (phospho S24) antibody
See all Transferrin Receptor products (58) ...
Description
Rabbit polyclonal to Transferrin Receptor (phospho S24)
Specificity
Detects endogenous levels of Transferrin Receptor only when phosphorylated at serine 24.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human Transferrin Receptor around the phosphorylation site of serine 24 (R-F-SP-L-A).
Positive control
Human brain tissue 293 cells, treated with PMA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Neural Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Non-lineage
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Post-translational
modifications
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP02786
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Database links
- Entrez Gene: 7037 Human
- Entrez Gene: 22042 Mouse
- Omim: 190010 Human
- SwissProt: P02786 Human
- SwissProt: Q62351 Mouse
- Unigene: 529618 Human
- Unigene: 28683 Mouse
Anti-Transferrin Receptor (phospho S24) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Transferrin Receptor (phospho S24) antibody (ab61021)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab61021 at a dilution of 1/50.
Western blot - Transferrin Receptor (phospho S24) antibody (ab61021)
All lanes : Anti-Transferrin Receptor (phospho S24) antibody (ab61021)
Lane 1 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins)
Lane 2 : extracts from 293 cells, treated
with PMA (125ng/ml, 30mins) with Immunizing phospho-peptide
Predicted band size : 85 kDa
Observed band size : 85 kDa
References for Anti-Transferrin Receptor (phospho S24) antibody (ab61021)
ab61021 has not yet been referenced specifically in any publications.
Publishing research using ab61021? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"