Anti-Transglutaminase 5/TGM5 antibody (ab133786)
Key features and details
- Rabbit polyclonal to Transglutaminase 5/TGM5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transglutaminase 5/TGM5 antibody
See all Transglutaminase 5/TGM5 primary antibodies -
Description
Rabbit polyclonal to Transglutaminase 5/TGM5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Transglutaminase 5/TGM5 (C terminal).
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Positive control
- Human spleen and liver tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes. -
Tissue specificity
Expressed in foreskin keratinocytes. -
Involvement in disease
Defects in TGM5 are a cause of peeling skin syndrome type A (APSS) [MIM:609796]. A non-inflammatory form of peeling skin syndrome, a genodermatosis characterized by the continuous shedding of the outer layers of the epidermis. In APSS patients, skin peeling is strictly limited to the dorsa of the hands and feet, and it is accompanied by accompanied by painless erythema and spontaneous non-scarring healing. Ultrastructural and histological analysis shows a level of blistering high in the epidermis at the stratum granulosum-stratum corneum junction. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Cellular localization
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 9333 Human
- Entrez Gene: 74176 Mouse
- Entrez Gene: 691929 Rat
- Omim: 603805 Human
- SwissProt: O43548 Human
- SwissProt: Q3V1F9 Mouse
- SwissProt: Q9D7I9 Mouse
- Unigene: 129719 Human
see all -
Alternative names
- Protein glutamine gamma glutamyltransferase 5 antibody
- Protein-glutamine gamma-glutamyltransferase 5 antibody
- PSS2 antibody
see all
Images
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Immunohistochemical analysis of formalin fixed, paraffin-embedded Human spleen tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 µg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemical analysis of formalin fixed, paraffin-embedded Human liver tissue labelling Transglutaminase 5/TGM5 with ab133786 at 5 µg/ml. Primary antibody labelling was followed by a biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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Datasheet download
References (2)
ab133786 has been referenced in 2 publications.
- Kapitány A et al. Regional Differences in the Permeability Barrier of the Skin-Implications in Acantholytic Skin Diseases. Int J Mol Sci 22:N/A (2021). PubMed: 34638769
- Medgyesi B et al. Rosacea Is Characterized by a Profoundly Diminished Skin Barrier. J Invest Dermatol 140:1938-1950.e5 (2020). PubMed: 32199994