Overview
Product nameTreponema Membrane Protein A proteinSee all Treponema Membrane Protein A proteins and peptides ...
Protein descriptionE.Coli derived recombinant fragment containing the Treponema Membrane Protein A immunodominant regions, 23-41 amino acids and 288-325 amino acids.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab68126 is purified by a proprietary chromatographic technique.
Biological activityab68126 is immunoreactive with sera of Treponema Pallidum infected individuals.
This protein binds to murine anti-TmpA protein monoclonal antibodies and Treponema pallidum converted human serum polyclonal antibodies in ELISA and Western Blot.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 8M Urea, 20mM DTT, 10mM Tris HCl, 1mM EDTA, pH 8
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68126
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
|
| WB |
|
| ELISA |
|
| Dot Blot |
|
Application notesDot: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
Lateral Flow immunochromatographic diagnostics test: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Antigen tmpAMembrane protein AtmpA
RelevanceTreponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled.
There is no vaccine for syphilis. The outer membrane of T. pallidum has too few surface proteins for an antibody to be effective. Efforts to develop a safe and effective syphilis vaccine have been hindered by uncertainty about the relative importance of humoral and cellular mechanisms to protective immunity and the fact that T. pallidum outer membrane proteins have not been unambiguously identified.
Cellular localizationCell Membrane
References for Treponema Membrane Protein A protein (ab68126)
ab68126
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
