Treponema pallidum p47 protein (ab51323)

Product Name 

Treponema pallidum p47 protein

Product type 

Proteins and Peptides

Peptide 

Recombinant fusion protein; Treponema pallidum p47, beta-galactosidase (114 kDa) fused at the N-terminal.

Specificity 

Reacts strongly with human T. pallidum positive serum.

Tested applications 
(see key)

ELISA, Flow Cyt, SDS-PAGE, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Other gram-ve bacteria

Relevance 

Treponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8

Material safety datasheet (MSDS) for this product:
Mercaptoethanol MSDS

Purification notes 

Purity of proteins is evaluated by SDS-PAGE, by measuring optical density at 280 nm and by method of Bradford et al.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Expression system 

E. coli

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