Overview

  • Product nameTriiodothyronine (T3) Human ELISA KitSee all Triiodothyronine (T3) kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low 20 1.37pg/ml 0.16 11.9%
    Normal 20 4.21pg/ml 0.17 4.1%
    High 20 7.1pg/ml 0.17 2.4%
    Low 20 1.37pg/ml 0.16 11.9%
    Normal 20 4.21pg/ml 0.17 4.1%
    High 20 7.1pg/ml 0.17 2.4%
  • Tests
    1 x 96 test
  • Sample type
    Serum
  • Assay typeCompetitive
  • Range
    0.9 pg/ml - 19 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.

    A 96-well plate has been pre-coated with Anti-Triiodothyronine antibodies. Samples, standards and the Triiodothyronine HRP Conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • Free T3
    • Triiodothyronine (T3)

Applications

Our Abpromise guarantee covers the use of ab108663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Triiodothyronine (T3) Human ELISA Kit images

  • Representative standard curve using ab108663

Protocols

References for Triiodothyronine (T3) Human ELISA Kit (ab108663)

ab108663 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"