Overview

  • Product nameTrkB Mouse ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell Lysate, Tissue Homogenate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    93.8 pg/ml - 6000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse TrkB in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse TrkB in cell culture supernatants, cell lysates and tissue homogenates.

    A TrkB specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkB is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TrkB amount of sample captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ligand-mediated auto-phosphorylation.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession Q16620 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • BDNF tropomyosine receptor kinase B
    • BDNF/NT-3 growth factors receptor
    • GP145-TrkB
    • Neurotrophic tyrosine kinase receptor type 2
    • Ntrk2
    • NTRK2_HUMAN
    • Trk-B
    • TRKB
    • TrkB tyrosine kinase
    • Tyrosine kinase receptor B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

TrkB Mouse ELISA Kit images

  • Representative standard curve using ab119610

Protocols

References for TrkB Mouse ELISA Kit (ab119610)

ab119610 has not yet been referenced specifically in any publications.

Product Wall

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Unfortunately, this kit cannot be used with rat samples.

We have 2 TrkB antibodies (ab51190 and ab43079) that have been tested in indirect ELISA and rat samples, and might work together in a sandwich EL...

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DISCOUNT CODE: ***
Expiration date: June 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab119609 in mouse. This code will give you 1 free ELISA kit before the expiration date. To redeem this offer, pleas...

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Thank you for your patience.

The lab informed me thatRIPA buffer is compatible with the kit and can be usedwithtissue.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"