TrkB Mouse ELISA Kit (ab119610)
- Product nameTrkB Mouse ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 5 pg/ml
- Range93.8 pg/ml - 6000 pg/ml
- Species reactivityReacts with: Mouse
- Product overview
ab119610 TrkB Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse TrkB specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TrkB amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
Store at 4°:C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well plate precoated with anti-mouse TrkB antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse TrkB antibody 1 x 130µl Lyophilized recombinant mouse TrkB standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- BDNF tropomyosine receptor kinase BBDNF/NT-3 growth factors receptorGP145-TrkB
- Neurotrophic tyrosine kinase receptor type 2Ntrk2NTRK2_HUMANTrk-BTRKBTrkB tyrosine kinaseTyrosine kinase receptor B
Our Abpromise guarantee covers the use of ab119610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TrkB Mouse ELISA Kit (ab119610)
ab119610 has not yet been referenced specifically in any publications.