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Overview

  • Product nameTrkC protein (Fc Chimera)See all TrkC proteins and peptides ...
  • Protein descriptionRecombinant fragment,extracellular region corresponding to amino acids 32-428 of Human TrkC
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQ DSGNSNGNANINITDISRNITSIHIENWRSLHTLN AVD MELYTGLQKLTIKNSGLRSIQPRAFAKNPHLRYINLSSNR LTTLSWQLFQTLSLRELQLE QNFFNCSCDIRWMQLWQE QGEAKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSH VNL TVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTH QTNLNWTNVHAINLTLVNVTSEDNGFTL TCIAENVVGM SNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNPPPT LHWLHNGQPLRE SKIIHVEYYQEGEISEGCLLFNKPTH YNNGNYTLIAKNPLGTANQTINGHFLKEPFPESTDNFIL FDEVSPTPPITVTHKPEEDGSSNTKVDKKVEPKSCDKTH TCPPCPAPELLGGPSVFLFPPKP KDTLMISRTPEVTCV VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRV VSVLTV LHQDWLNGKEYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSRDELTKNQVSLTCLVK GFYPSDIA VEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMHE ALHNHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab85599 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab85599 migrates as a broad band between 85 and 150 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 71.9 kDa.

    pI: ab85599 separates into a number of isoforms with a pI between 4 and 7 in 2D PAGE due to post-translational modifications, in particular glycosylation. This
    compares with the unmodified protein that has a predicted pI of 5.8.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        EC 2.7.10.1ETS related protein neurotrophic receptor tyrosine kinase fusionETV6 NTRK3 fusion
        GP145 TrkCgp145(trkC)GP145-TrkCGP145TrkCNeurotrophic tyrosine kinase receptor type 3Neurotrophin 3 receptorNT 3 growth factor receptorNT 3 growth factor receptor precursorNT 3 receptorNT-3 growth factor receptorNTRK3NTRK3_HUMANOTTHUMP00000192915TRK CTrk-CTrkC tyrosine kinaseTyrosine kinase receptor C
      see all
  • FunctionReceptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
  • Tissue specificityWidely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Ligand-mediated auto-phosphorylation.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession Q16288 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TrkC protein (Fc Chimera) images

    • SDS-PAGE - TrkC protein (Fc Chimera) (ab85599)
      SDS-PAGE - TrkC protein (Fc Chimera) (ab85599)
      1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab85599
      Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
      Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
      5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.

    • Functional Studies - TrkC protein (Fc Chimera) (ab85599)
      Functional Studies - TrkC protein (Fc Chimera) (ab85599)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.

    References for TrkC protein (Fc Chimera) (ab85599)

    ab85599 has not yet been referenced specifically in any publications.

    Publishing research using ab85599 ? Please let us know so that we can cite the reference in this datasheet

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