TrkC protein (Fc Chimera) (ab85599)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab85599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Molecular Mass: ab85599 migrates as a broad band between 85 and 150 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 71.9 kDa.
pI: ab85599 separates into a number of isoforms with a pI between 4 and 7 in 2D PAGE due to post-translational modifications, in particular glycosylation. This
compares with the unmodified protein that has a predicted pI of 5.8.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- EC 126.96.36.199ETS related protein neurotrophic receptor tyrosine kinase fusionETV6 NTRK3 fusion
- GP145 TrkCgp145(trkC)GP145-TrkCGP145TrkCNeurotrophic tyrosine kinase receptor type 3Neurotrophin 3 receptorNT 3 growth factor receptorNT 3 growth factor receptor precursorNT 3 receptorNT-3 growth factor receptorNTRK3NTRK3_HUMANOTTHUMP00000192915TRK CTrk-CTrkC tyrosine kinaseTyrosine kinase receptor C
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain.
TrkC protein (Fc Chimera) images
1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab85599
Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
References for TrkC protein (Fc Chimera) (ab85599)
ab85599 has not yet been referenced specifically in any publications.