Trypsin Enzymatic Antigen Retrieval Solution (ab970)
- Product nameTrypsin Enzymatic Antigen Retrieval SolutionSee all Antigen retrieval solution reagents ...
- Tested applicationsIHC-P more details
- General notesProtocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8ºC). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded buffer (rose) for easy identification. The buffer incorporates ASSURE™ Technology, a color-coded pH indicator. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, Carrier protein, pH 7.3
- Concentration information loading...
- Reagent notes Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
- Research Areas
Our Abpromise guarantee covers the use of ab970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.|
References for Trypsin Enzymatic Antigen Retrieval Solution (ab970)
This product has been referenced in:
- Williams CD et al. Acetaminophen-induced hepatic neutrophil accumulation and inflammatory liver injury in CD18-deficient mice. Liver Int : (2010). Read more (PubMed: 20500806) »
- Graham SA et al. Prolectin, a glycan-binding receptor on dividing B cells in germinal centers. J Biol Chem 284:18537-44 (2009). Read more (PubMed: 19419970) »