Trypsin Enzymatic Antigen Retrieval Solution (ab970)

Overview

  • Product nameTrypsin Enzymatic Antigen Retrieval Solution
    See all Antigen retrieval solution reagents
  • Tested applicationsIHC-Pmore details
  • General notesProtocol Notes: 1) Deparaffinize tissues and hydrate to water. If necessary perform a hydrogen peroxide block, wash in water, and rinse in PBS or TBS. The tissue section is ready for trypsin digestion. 2) Combine 1 part trypsin concentrate with 1 part buffer (working solution stable for 5 working days at 2-8ºC). Incubate section for 5-10 minutes at 37°C or 15 minutes at room temperature. Then, rinse well in PBS or TBS buffer and proceed with immunostaining procedure. This product utilises a color-coded buffer (rose) for easy identification. The buffer incorporates ASSURE™ Technology, a color-coded pH indicator. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion.

Properties

  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, Carrier protein, pH 7.3
  • Concentration information loading...
  • Reagent notes Trypsin is a commonly used digestive enzyme. In formalin-fixed, paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab970 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use at an assay dependent dilution. Ratio for 25ml concentrated trypsin and 25 ml buffer is 1:1 - 1:3.

    References for Trypsin Enzymatic Antigen Retrieval Solution (ab970)

    This product has been referenced in:
    • Williams CD  et al. Acetaminophen-induced hepatic neutrophil accumulation and inflammatory liver injury in CD18-deficient mice. Liver Int : (2010). Read more (PubMed: 20500806) »
    • Graham SA  et al. Prolectin, a glycan-binding receptor on dividing B cells in germinal centers. J Biol Chem 284:18537-44 (2009). Read more (PubMed: 19419970) »

    See all 4 Publications for this product

    Product Wall


    There are 2X 25 ml vials in the kit

    1 vial is 25 ml Trysin (concentrated liquid)

    2nd vial 25 ml Trypsin buffer

    Thank you for contacting us.

    For a standard IHC-P protocol I usually recommend a HIER method (Heat Induced Epitope Retrieval) for the antigen retrieval step using a Tris/EDTA pH 9.0 buffer as described in section "D" of the followi...

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    Thank you for contacting us. Yes, ab970 comes with 25 mL of concentrated Trpsin and 25 mL of Trypsin buffer.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    U...

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    Thank you for your email.

    To answer your questions and make some comments/suggestions that hopefully will help you further:

    1) The antibodies (shipped as is) should be stored aliquoted and stored at -20 or -80 degree. This would als...

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    Thank you for your email.
    I am sorry to hear the antigen retrieval step did not improve your results.

    I have looked throught your protocol you sent me and have the following suggestions, comments and questions:

    1) You are makin...

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    Thank you for your reply.
    I'm glad to hear that the secondary antibody shows fluorescence.

    As for selecting another secondary antibody, could you please let me know which fluorophore or wavelength range is covered by your miscroscope? Ba...

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    Thank you for your email.
    I would recommed one of the following trypsin products:
    ab970: Trypsin Enzymatic Antigen Retrieval Solution
    http://www.abcam.com/trypsin-enzymatic-antigen-retrieval-solution-ab970.html
    ab64205: Trypsin Enzy...

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    Thank you for contacting us.
    I am sorry to hear that the antibodie might not be working as expected.

    As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem:

    1) Try antig...

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    Thank you for contacting Abcam. For ab6328, I would recommend using ab970, the Trypsin Enzymatic Antigen Retrieval Solution that you mentioned. I believe that this will give you very good results in your IHC staining. For ab1416, from what I have read,...

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    Thank you for your reply. I apologize for this inconvenience, but we do not sell kit components separately so the buffer is not available as a stand-alone item. How much extra trypsin do you have? It might be worth trying to dilute in the calcium chlor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"