Trypsin Enzymatic Pretreatment Kit (ab64205)

Overview

  • Product nameTrypsin Enzymatic Pretreatment Kit
  • Product overview

    Trypsin is a digestive enzyme commonly used in formalin-fixed, paraffin-embedded immunohistochemical procedures. Its digestive properties are in many cases required as a pretreatment, prior to the primary antibody application, to produce a signal with certain protein-antibody interactions.

  • Notes

    Trypsin is a digestive enzyme commonly used in formalin-fixed, paraffin-embedded immunohistochemical procedures. Its digestive properties are in many cases required as a pretreatment, prior to the primary antibody application, to produce a signal with certain protein-antibody interactions.

  • Tested applicationsIHC-P more details

Properties

  • RelevanceTrypsin is a member of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.
  • Cellular localizationSecreted, extracellular space.
  • Alternative names
    • Mesotrypsin
    • MTG
    • Protease serine 1
    • Protease serine 2
    • Protease serine 3
    • Protease serine 4
    • PRSS 1
    • PRSS 2
    • PRSS 3
    • PRSS 4
    • PRSS1
    • PRSS2
    • PRSS3
    • PRSS4
    • Serine protease 1
    • Serine protease 2
    • Serine protease 3
    • TRP 1
    • Trypsin 1
    • Trypsin 2
    • Trypsin 3
    • Trypsin I
    • Trypsin II
    • Trypsin III
    • Trypsin IV
    • Trypsinogen 1
    • Trypsinogen 2
    • Trypsinogen 4
    see all

Applications

Our Abpromise guarantee covers the use of ab64205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.

References for Trypsin Enzymatic Pretreatment Kit (ab64205)

ab64205 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.
I would recommed one of the following trypsin products:
ab970: Trypsin Enzymatic Antigen Retrieval Solution
http://www.abcam.com/trypsin-enzymatic-antigen-retrieval-solution-ab970.html
ab64205: Trypsin Enzy...

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Thank you for contacting us.
I am sorry to hear that the antibodie might not be working as expected.

As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem:

1) Try antig...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"