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Trypsin Enzymatic Pretreatment Kit (ab64205)

Q&A (2)

Overview

  • Product nameTrypsin Enzymatic Pretreatment Kit
  • Product overview

    Trypsin is a digestive enzyme commonly used in formalin-fixed, paraffin-embedded immunohistochemical procedures. Its digestive properties are in many cases required as a pretreatment, prior to the primary antibody application, to produce a signal with certain protein-antibody interactions.

  • Notes

    Trypsin is a digestive enzyme commonly used in formalin-fixed, paraffin-embedded immunohistochemical procedures. Its digestive properties are in many cases required as a pretreatment, prior to the primary antibody application, to produce a signal with certain protein-antibody interactions.

  • Tested applicationsIHC-P more details

Properties

  • RelevanceTrypsin is a member of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.
  • Cellular localizationSecreted, extracellular space.
    • Alternative names
        MesotrypsinMTGProtease serine 1
        Protease serine 2Protease serine 3Protease serine 4PRSS 1PRSS 2PRSS 3PRSS 4PRSS1PRSS2PRSS3PRSS4Serine protease 1Serine protease 2Serine protease 3TRP 1Trypsin 1Trypsin 2Trypsin 3Trypsin ITrypsin IITrypsin IIITrypsin IVTrypsinogen 1Trypsinogen 2Trypsinogen 4
      see all

    Applications

    Our Abpromise guarantee covers the use of ab64205 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P

    References for Trypsin Enzymatic Pretreatment Kit (ab64205)

    ab64205 has not yet been referenced specifically in any publications.

    Publishing research using ab64205 ? Please let us know so that we can cite the reference in this datasheet

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    For this antibody (75693), antigen retrieval is advised with trypsin: is there a brand/lot that your recommend of trypsin?

    Thank you for your email.
    I would recommed one of the following trypsin products:
    ab970: Trypsin Enzymatic Antigen Retrieval Solution
    http://www.abcam.com/trypsin-enzymatic-antigen-retrieval-solution-ab970.html
    ab64205: Trypsin Enzymat...

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    IHC-Fr with adult rat brain (hippocampus)
    no signal at all
    20 um sections
    fixation: PFA 4% overnight
    Ab: ab6994: 1/200, 1/500, 1/800 o/n 4 degree
    ab104225: 1/500, 1/1000, 1/2000 o/n 4 degree
    block: none
    secondary: 1/200 ...

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    Thank you for contacting us.
    I am sorry to hear that the antibodie might not be working as expected.

    As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem:

    1) Try antigen ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"