Anti-Tryptophan Hydroxylase antibody (ab78969)

Overview

• Product nameAnti-Tryptophan Hydroxylase antibodySee all Tryptophan Hydroxylase primary antibodies ...
• Description
  Rabbit polyclonal to Tryptophan Hydroxylase
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic non-phosphopeptide derived from human Tryptophan Hydroxylase around the phosphorylation site of serine 260 (H-S-S^P-D-P).

• Positive controlHuman lung carcinoma tissue. Extracts from Jurkat cells. HeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Endocrine metabolism
  • Hormone biosynthesis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Neuroscience
  • Neurotransmitter
  • Amino Acids
  • Tryptophan

Applications

Target

• Tissue specificityIsoform 2 seems to be less widely expressed than isoform 1.
• PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
• Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  Contains 1 ACT domain.

Target information above from: UniProt accession P17752 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7166 Human
  • Entrez Gene: 7166 Human
  • Entrez Gene: 21990 Mouse
  • Entrez Gene: 24848 Rat
  • Omim: 191060 Human
  • SwissProt: P17752 Human
  • SwissProt: P17752 Human
  • SwissProt: P17532 Mouse

  • SwissProt: P09810 Rat
  • Unigene: 591999 Human
  • Unigene: 248684 Mouse
  • Unigene: 94788 Rat

  see all

• Alternative names
  Indoleacetic acid 5 hydroxylase antibodyL tryptophan hydroxylase antibodyMGC119994 antibody

  TPH 1 antibodyTPH antibodyTPH1 antibodyTPH1_HUMAN antibodyTPRH antibodyTRPH antibodyTryptophan 5 hydroxylase 1 antibodyTryptophan 5 monooxygenase 1 antibodyTryptophan 5 monooxygenase antibodyTryptophan 5-hydroxylase 1 antibodyTryptophan 5-monooxygenase 1 antibodyTryptophan hydroxylase 1 antibody

  see all

Anti-Tryptophan Hydroxylase antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tryptophan Hydroxylase antibody (ab78969)
  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab78969 at 1/50 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
• 

  Immunocytochemistry/ Immunofluorescence - Tryptophan Hydroxylase antibody (ab78969)
  Immunofluorescence analysis of HeLa cells, using ab78969 at 1/500 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
• 

  Western blot - Tryptophan Hydroxylase antibody (ab78969)
  All lanes : Anti-Tryptophan Hydroxylase antibody (ab78969) at 1/500 dilution
  
  Lane 1 : Extracts from Jurkat cells
  Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 51 kDa
  Observed band size : 51 kDa