Overview

  • Product name
    Anti-Tryptophanyl tRNA synthetase antibody
    See all Tryptophanyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Tryptophanyl tRNA synthetase
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human): Tryptophanyl tRNA synthetase

  • Positive control
    • human normal breast tissue. HEK293 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab31536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/50 - 1/1000.
IP Use at an assay dependent concentration.

Target

  • Function
    Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
  • Sequence similarities
    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Post-translational
    modifications
    Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma 2 antibody
    • GAMMA-2 antibody
    • Gamma2 antibody
    • hWRS antibody
    • IFI 53 antibody
    • IFI53 antibody
    • IFP 53 antibody
    • IFP53 antibody
    • Interferon induced protein 53 antibody
    • Interferon-induced protein 53 antibody
    • SYWC_HUMAN antibody
    • T2-TrpRS antibody
    • TrpRS antibody
    • Tryptophan tRNA ligase, cytoplasmic antibody
    • Tryptophan tRNA ligase 1 cytoplasmic antibody
    • Tryptophan tRNA ligase antibody
    • Tryptophan--tRNA ligase antibody
    • Tryptophanyl tRNA synthetase antibody
    • Tryptophanyl tRNA synthetase cytoplasmic antibody
    • WARS antibody
    • WARS protein antibody
    • WRS antibody
    see all

Images

  • Anti-Tryptophanyl tRNA synthetase antibody (ab31536) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab31536 staining Tryptophanyl tRNA synthetase in HeLa cells by Immunocytochemistry/ Immunofluorescence.

References

This product has been referenced in:
  • Norris EL  et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . Read more (PubMed: 27451424) »
  • Al-Gubory KH  et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction 147:599-614 (2014). WB, IHC-P ; Sheep . Read more (PubMed: 24446454) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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