Primary antibodies
- A-Z
- Primary antibodies
- Tags and cell markers
- Antibody Panels
- Chromatin
- Cancer
- Immunology
- Stem Cells
- By research area
- Cancer
- Cardiovascular
- Cell Biology
- Epigenetics & Nuclear Signaling
- Developmental Biology
- Immunology
- Metabolism
- Microbiology
- Neuroscience
- Signal Transduction
- Stem Cells
Secondary antibodies
- Fragment antibodies
- F(ab) secondary antibodies
- F(ab')2 secondary antibodies
- Resources
- How to choose a secondary antibody
- How to choose a conjugate
- Advantages of F(ab) and F(ab')2 fragments
- Learn more about DyLights®
- Learn more about pre-adsorbtion
- Advanced search
Proteins & Peptides
- A-Z
- Full length proteins
- Partial proteins
- Peptides
- Enzymes
- MMPs
- Caspases
- Kinases and transferases
- Signal transduction related
- Cytokines and growth factors
- Cytokines
- Growth factors by family
- Human proteins
- Human expressed
- Barley expressed
- Wheat expressed
- Fc fusion proteins
- Ig proteins
- His-tagged Proteins by research area
- Cancer
- Cardiovascular
- Epigenetics & Nuclear Signaling
- Cell Biology
- Immunology
- Neuroscience
- Signal Transduction
- Stem Cells
- Microbiology and virology
- All microbiology and viral proteins
- DNA virus proteins
- RNA viral proteins
- All Hepatitis virus proteins
- HIV proteins
- HSV, Measles and Influenza proteins
- Bacterial toxins and chaperones
- Resources
- Advantages of expression in barley
ELISA Kits
- A-Z
- ELISA Kits
- ELIPAIR Kits
- ELISPOT Kits
- FLUOROSPOT Kits
- ELISA development
- Matched antibody pairs
- Resources
- Immunoassay technical know-how
- ELISA and ELIPAIR
- ELISPOT and FLUOROSPOT
Assay Kits
- A-Z
- Apoptosis kits
- Epigenetic kits
- Metabolism kits
- ChIP kits
- Metabolism kits by analyte
- Alcohol
- Amino acids
- Calcium
- Cell viability and senescence
- Coenzymes and cofactors
- Creatinine
- Inorganic ions
- Intermediary metabolism
- Lipid metabolism
- Nitric oxide
- Phosphates
- Sugars
- Oxidative stress
- Other metabolism assays
- Apoptosis kits by analyte
- Annexin V
- Calpain
- Caspases
- Cathepsins
- DNA fragmentation
- Transmembrane potential
- Other Apoptosis assays
- Epigenetic Kits by modification
- DNA modification and methylation
- Histone demethylation
- Histone methylation activity
- Sample Preparation
- Histone methylation quantification
- Histone acetylation and deacetylation
- Histone phosphorylation
- Sumoylation
Other Kits
- A-Z
- All kits
- Antibody labeling
- EasyLink antibody conjugation kits
- Cell fractionation
- Cell fractionation kits A-Z
- Cell Staining Kits
- CytoPainter kits
Biochemicals
- A-Z
- By pharmacology
- By research area
- By chemical type
- By product range
- Receptors & Transporters
- Adenosine
- Adrenoceptors
- Cannabinoid
- Catalytic Receptors
- Dopamine
- GABA
- Glutamate
- Glycine
- Histamine
- Imidazoline
- Melatonin
- Muscarinic
- Nicotinic
- Nuclear Receptors
- Opioids
- Peptide Receptors
- Purinergics
- Serotonin
- Transporters
- Ion channels
- All ion channel modulators
- Enzymes
- All enzyme modulators
- Signaling
- Amyloid peptides
- Apoptosis & cell cycle
- Ca²⁺ signaling
- Coenzymes
- Cytoskeleton & motor proteins
- DNA, RNA & protein synthesis
- Endocytosis & exocytosis
- Hedgehog signaling
- Immunomodulators
- Lipid signaling
- Nitric Oxide
Immunodetection
- IHC Kits
- EXPOSE biotin-free detection kits
- Biotin-Streptavidin detection kits
- Endogenous avidin/biotin blocking systems
- Substrate kits
- Mouse on Mouse detection kit
- BrdU IHC detection kit
- Secondary antibodies
- Advanced search
- Reagents and buffers
- Buffers
- Chromogens and enhancers
- Diluents
- Epitope recovery and pretreatments
- Fluorescent ligands
- Mounting media
- PAP pen
- Serum
- Substrates
- Tissue Slides
- All Human
- Human adult (normal)
- Human fetal
- Human tumor
- Mouse
- Western Blot reagents
- ECL kits
- Optiblot buffers and stains
- Optiblot electrophoresis gels
- Optiblot electrophoresis kits
- Protein ladders
- Protein quantification kits
- Text books
- Immunohistochemistry - Methods Express Book Series
Controls
- A-Z
- Isotype controls
- Loading controls
- Lysates
- Isotype controls by host species
- Chicken
- Goat
- Hamster (Armenian and Syrian)
- Mouse
- Rabbit
- Rat
- Sheep
- Lysates by type
- 293T transfected
- Cytoplasmic
- Membrane
- Nuclear
- Tissue
- Total protein
- Whole cell
- Loading controls
- Loading controls by protein target

Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules

Anti-Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

55Five stars 4 Abreviews

1 Specific reference

Tweet
Share email

Code	Size	Price	Abpoints	Availability
				Add to basket

Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Specific protocols

To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on.

General protocols

Useful resources:

Western blotting (WB) protocols:

Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:

Chromatin Immunoprecipitation (ChIP) protocols:

Dot blot protocols:

Dot Blot protocol

ELISA protocols:

ELISPOT protocols:

Flow cytometry / FACS protocols:

Immunoprecipitation (IP) protocols:

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Immunocytochemistry/ Immunofluorescence - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

ab11323 at 1/800 staining dog kidney cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-mouse IgG was used as the secondary.

This image is courtesy of an anonymous Abreview

Western blot - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

All lanes : Anti-Tubulin (acetyl K40) antibody [6-11B-1] (ab11323) at 1/1000 dilution

Lane 1 : Whole animal lysate prepared from wild type (N2) C. Elegans worms.
Lane 2 : Whole animal lysate prepared from elpc-1 C. Elegans worms.
Lane 3 : Whole animal lysate prepared from elpc-3 C. Elegans worms.
Lane 4 : Whole animal lysate prepared from mec-12 (e1605) C. Elegans worms.
Lane 5 : Whole animal lysate prepared from mec-12 (e1607) C. Elegans worms.

Lysates/proteins at 20 µg per lane.

To measure acetylated α-tubulin levels by western blot, protein was extracted from young adult worms. To avoid protein degradation, worms were suspended in ice-cold extraction buffer containing proteinase inhibitors and rapidly frozen in liquid nitrogen. The frozen pellets were ground to a powder in a mortar.

Image from Chen C et al, PLoS Genet. 2009 Jul;5(7):e1000561. Epub 2009 Jul 10, fig S9.

Western blot - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

Anti-Tubulin (acetyl K40) antibody [6-11B-1] (ab11323) at 1/2000 dilution (in PBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human IHP1 cells at 30 µg

Secondary
An HRP-conjugated Goat anti-mouse IgG monoclonal at 1/2000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Exposure time : 20 seconds

Blocking Step: 5% Milk for 2 hours at 22°C

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

ab11323 staining Tubulin (acetyl K40) in human glioblastoma tumor sphere cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4ºC. An Alexa Fluor^®488-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

This image is courtesy of an abreview submitted by Lars Ruether

Tubulin (acetyl K40) antibody [6-11B-1] for Western blot in Human (11323)

Tubulin (acetyl K40) antibody [6-11B-1] for Western blot in Mouse (11323)

Tubulin (acetyl K40) antibody [6-11B-1] for ICC/IF in Dog (11323)

Tubulin (acetyl K40) antibody [6-11B-1] for Immunocytochemistry/ Immunofluorescence in Human (11323)

A-Z

Antibody Panels

By research area

A-Z

DyLight Conjugated

Fragment antibodies

Resources

A-Z

Enzymes

Cytokines and growth factors

Human proteins

His-tagged Proteins by research area

Microbiology and virology

Resources

A-Z

ELISA development

Resources

ELISA Kits by protein family

A-Z

Metabolism kits by analyte

Apoptosis kits by analyte

Epigenetic Kits by modification

A-Z

Antibody labeling

Cell fractionation

Cell Staining Kits

IHC Kits

A-Z

Receptors & Transporters

Ion channels

Enzymes

Signaling

IHC Kits

Secondary antibodies

Reagents and buffers

Tissue Slides

Western Blot reagents

Text books

A-Z

Isotype controls by host species

Lysates by type

Loading controls

Anti-Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

-

Product partner

Reassurance, Refunds & Replacements

Specific protocols

General protocols

Useful resources:

Western blotting (WB) protocols:

Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:

Chromatin Immunoprecipitation (ChIP) protocols:

Dot blot protocols:

ELISA protocols:

Flow cytometry / FACS protocols:

Immunoprecipitation (IP) protocols:

Immunocytochemistry/ Immunofluorescence - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

Western blot - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

Western blot - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

Immunocytochemistry/ Immunofluorescence - Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

A-Z by research area

A-Z by product type

Help & support

Company

Events

Talk to us

Special offers