Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

Product Name 

Tubulin (acetyl K40) antibody [6-11B-1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [6-11B-1] to Tubulin (acetyl K40)

Immunogen 

Acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin)

Reacts with 
(species key)

Hu, Ms, Rat, Ce, Chk, C rei, Cow, Dog, Hm, Pig, S urc, Xl

Tested applications 
(see key)

Dot, ELISA, ICC/IF, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: 1/2000. This dilution was determined using bovine or rat brain preparation.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules

Relevance 

The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells.
Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues.
Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6-11B-1

Isotype 

IgG2b

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Ascites fluid

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 1.30 - 1.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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References for Tubulin (acetyl K40) antibody [6-11B-1] (ab11323)

  This product has been used in:

Chen C et al. Defects in tRNA modification associated with neurological and developmental dysfunctions in Caenorhabditis elegans elongator mutants. PLoS Genet 5:e1000561 (2009). WB; Caenorhabditis elegans. PubMed: 19593383

If you publish research using ab11323 please let us know so that we can cite the reference on this datasheet.

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