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Product Name
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Tubulin antibody [B3] - Polyglutamylated
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See all Tubulin antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B3] to Tubulin - Polyglutamylated
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Immunogen
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Purified sea urchin (Lytechinus pictus) sperm axonemal proteins.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Mcq, Rb, S urc
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Epitope
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The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubulins (the glutamylated motif at amino acid 445-457 of alpha tubulin).
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Tested applications
(see key)
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ELISA, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution. Can be used with 4% paraformaldehyde-1% Triton X-100 and methanol-acetone.
WB: Use at a concentration of 0.5 - 1 µg/ml. This concentration is recommended using a cytosolic fraction of rat brain extract. Detects a band of approximately 50 kDa, possibly a doublet of alpha and beta tubulins.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubulins (the glutamylated motif at amino acid 445-457 of alpha tubulin).
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells.
Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues.
Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B3
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Isotype
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IgM
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Purity
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Protein A purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.100 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilutions should be discarded if not used within 12 hours.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Tubulin antibody [B3] - Polyglutamylated is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Tubulin antibody [B3] - Polyglutamylated (ab11324)
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This product has been used in: (two most recent references)
Keller LC et al. Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length control. Mol Biol Cell 20:1150-66 (2009). ICC/IF; Human. PubMed: 19109428
van Dijk J et al. A targeted multienzyme mechanism for selective microtubule polyglutamylation. Mol Cell 26:437-48 (2007). PubMed: 17499049
See all 3 publication references for this product.
If you publish research using ab11324 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Tubulin
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