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Anti-Tubulin antibody [TUB-1A2] (ab11325)

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Overview

Product name

Anti-Tubulin antibody [TUB-1A2]
See all Tubulin products (8) ...

Description

Mouse monoclonal [TUB-1A2] to Tubulin

Tested applications

WB, IFmore details

Cross reactivity

Reacts with

Cow

Immunogen

Synthetic peptide containing the carboxy terminal amino acids of alpha tubulin.

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

TUB-1A2

Isotype

IgG3

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     
  • IF

     

Application notes

IF: 1/800. This concentration was determined for cultured chicken fibroblasts.
WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Sequence similarities

Belongs to the tubulin family.

Post-translational
modifications

Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Cellular localization

Cytoplasm > cytoskeleton.

Target information above from: UniProt accessionP68363 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alpha tubulin antibody
  • Alpha-tubulin ubiquitous antibody
  • Beta 5 tubulin antibody
  • Beta tubulin antibody
  • DAQB 47P19.9 001 antibody
  • dJ40E16.7 antibody
  • K alpha 1 antibody
  • K alpha 1 protein antibody
  • M40 antibody
  • MGC117247 antibody
  • MGC16435 antibody
  • OK/SW cl.56 antibody
  • TBA1B_HUMAN antibody
  • TUBA1 antibody
  • TUBA1B antibody
  • TUBB 1 antibody
  • TUBB 2 antibody
  • TUBB 3 antibody
  • TUBB 4 antibody
  • TUBB 5 antibody
  • TUBB antibody
  • TUBB protein antibody
  • TUBB1 antibody
  • TUBB2 antibody
  • TUBB2A antibody
  • TUBB2C antibody
  • TUBB3 antibody
  • TUBB4 antibody
  • TUBB5 antibody
  • Tubulin alpha-1B chain antibody
  • Tubulin alpha-ubiquitous chain antibody
  • Tubulin beta 1b antibody
  • Tubulin beta 2 chain antibody
  • Tubulin beta 2A antibody
  • Tubulin beta antibody
  • Tubulin beta polypeptide antibody
  • Tubulin K-alpha-1 antibody
  • XXbac BCX111D4.2 001 antibody
see all

Database links

References for Anti-Tubulin antibody [TUB-1A2] (ab11325)

ab11325 has not yet been referenced specifically in any publications.

Publishing research using ab11325? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"