Tubulin antibody [TUB-1A2] (ab11325)

Product Name 

Tubulin antibody [TUB-1A2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [TUB-1A2] to Tubulin

Immunogen 

Synthetic peptide containing the carboxy terminal amino acids of alpha tubulin.

Reacts with 
(species key)

Cow

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IF: 1/800. This concentration was determined for cultured chicken fibroblasts.
WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules

Relevance 

The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells.
Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues.
Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

TUB-1A2

Isotype 

IgG3

Purity 

Ascites

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Ascites fluid.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 6.80 - 6.80 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

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