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- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161_1.jpg)
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- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161_2.jpg)
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- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161_31.jpg)
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Product Name
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Tubulin antibody [YOL1/34] - Microtubule Marker
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See all Tubulin antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
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Immunogen
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Full length native protein (purified) (S. cerevisiae).
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Reacts with
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Hu, Ms, Rat, Allg, Dog, Sc, S pom
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Predicted to react
(species key)
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Many, Mamm, Plnts
(Due to sequence homology)
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Specificity
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This antibody detects an epitope on alpha-tubulin between the amino acids 414-422. It has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-FoFr, RIA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/4000.
ICC/IF: Use at an assay dependent dilution from (PubMed:17316608).
IHC-Fo: Suggested working dilution of 1/600 (See PMID: 15831501).
RIA: Use at an assay dependent dilution.
WB: 1/100 - 1/3000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Ab6161 binds to an epitope between amino acids 414 and 422 of alpha
tubulin.
This antibody gave a positive signal in the following lysates:
HeLa Whole Cell, Rat Brain Tissue, NIH3T3 Whole Cell
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells.
Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues.
Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms.
Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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YOL1/34
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Isotype
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IgG2a
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Tubulin antibody [YOL1/34] - Microtubule Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Tubulin antibody [YOL1/34] - Microtubule Marker images, references, products related to ab6161 and other tools.
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Tubulin antibody [YOL1/34] - Microtubule Marker images:
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 - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
Western blot against tubulin with ab6161 at 1/3000. Secondary Rabbit anti-Rat IgG HRP (ab6734)was used at 1/2000. Exposure time: 2mins.
Lane 1: 20µg/lane HeLa (Human) whole cell lysates (ab7898).
Lane 2: 20µg/lane 3T3 (Mouse) whole cell lysate (ab7901).
Lane 3: 20µg/lane Rat brain tissue lysate (ab7942).
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- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
Cultured human macrophages were used with ab6161 at 1/1000 for immunofluorescence. Cells were fixed with cold 2% formaldehyde for 20mins.
Green staining is Alexa 568, Blue staining is DAPI stain.
This cell represents a young macrophage, the staining patterns varied as the cells aged in culture.
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- Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
Confocal image of 21 day in vitro rat hippocampal neurons, stained with rat monoclonal antibody to Tubulin - Microtubule Marker (ab6161) in green at 1/500 and Microtubule Associated protein 2 in blue.
This picture was kindly supplied as part of the review submitted by Dr Jonathon Burman.
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Western blot - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![Western blot - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161_5.jpg)
All lanes : Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1/2000 dilution
Lane 1 : Yeast whole cell extract prepared by bead-beating
Lane 2 : Yeast whole cell extract prepared by bead-beating
Lane 3 : Yeast whole cell extract prepared by bead-beating
Lane 4 : Yeast whole cell extract prepared by bead-beating
Lane 5 : Yeast whole cell extract prepared by bead-beating
Lane 6 : Yeast whole cell extract prepared by bead-beating
Lane 7 : Yeast whole cell extract prepared by bead-beating
Lane 8 : Yeast whole cell extract prepared by bead-beating
Lane 9 : Yeast whole cell extract prepared by bead-beating
Lane 10 : Yeast whole cell extract prepared by bead-beating
Lysates/proteins at 5 µg per lane.
Secondary
HRP conjugated goat anti-rat antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![Immunocytochemistry/ Immunofluorescence - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161-IF_Im1.jpg)
ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
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Immunocytochemistry/ Immunofluorescence - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![Immunocytochemistry/ Immunofluorescence - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/ab6161_6.jpg)
ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
![Western blot - Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)](/ps/datasheet/images/6/ab6161/Tubulin-Primary-antibodies-ab6161-10.jpg)
Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
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References for Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
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This product has been used in: (two most recent references)
Soubry A et al. The transcriptional repressor kaiso localizes at the mitotic spindle and is a constituent of the pericentriolar material. PLoS One 5:e9203 (2010). ICC/IF; Human. PubMed: 20169156
Stengel C et al. Class III beta-tubulin expression and in vitro resistance to microtubule targeting agents. Br J Cancer 102:316-24 (2010). WB; Human. PubMed: 20029418
See all 32 publication references for this product.
If you publish research using ab6161 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Tubulin
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