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Anti-Tubulin antibody (ab59680)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab59680 for help.

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2 questions for ab59680

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Question 1

Tuesday 15-July-2008

The answer I received is still not specific enough. I wanted to know if it was based on a Tris Glycine gel or some other type of SDS-Page gel type.

ANSWER:

  

Thank you for your enquiry.

I have recontacted the originator for detail information and they have confirmed that a Tris Glycine gel was used in the WB.

If there is anything else that I can help you with, please do not hesitate to contact us.

Have a nice day.

Question 2

Wednesday 09-April-2008

When you show an image of a western blot band, is the electrophoresis done on Tris Glycine gels? I'm concerned about the MW assignment (in this case 55kD) and I need to be sure it's based on TG gels otherwise it might migrate with my protein of interest (LDH-A, lactate dehydrogenase A), which runs just a tad lower than GAPDH. Thanks

ANSWER:

  

Thank you for your enquiry.

I have confirmed with the originator of the product that the WB testing was performed using a SDS-PAGE gel.

I hope this information will not hinder your experimental results. If there is anything else that I can help you with, please do not hesitate to contact me.

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