Anti-Tyrosine Hydroxylase antibody [TOH A1.1] (ab150659)
Key features and details
- Mouse monoclonal [TOH A1.1] to Tyrosine Hydroxylase
- Suitable for: IHC-Fr, ICC/IF, Electron Microscopy
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody [TOH A1.1]
See all Tyrosine Hydroxylase primary antibodies -
Description
Mouse monoclonal [TOH A1.1] to Tyrosine Hydroxylase -
Host species
Mouse -
Specificity
The antibody recognizes the full length TOH
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Tested applications
Suitable for: IHC-Fr, ICC/IF, Electron Microscopymore details -
Species reactivity
Reacts with: Rat -
Immunogen
Full length native Rat Tyrosine Hydroxylase protein (purified).
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General notes
ab150659 reacts with tyrosine hydroxylase immunoreactive sites in the known catecholaminergic neurons and and fibre systems of rat brain. It recognizes best the configuration of tyrosine hydroxylase in fixed tissues.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: 99% Water -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Lyophilized protein G purified in PBS pH7.4 -
Primary antibody notes
ab150659 reacts with tyrosine hydroxylase immunoreactive sites in the known catecholaminergic neurons and and fibre systems of rat brain. It recognizes best the configuration of tyrosine hydroxylase in fixed tissues. -
Clonality
Monoclonal -
Clone number
TOH A1.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Electron Microscopy |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Electron Microscopy
Use at an assay dependent concentration. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 25085 Rat
- SwissProt: P04177 Rat
- Unigene: 11082 Rat
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Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab150659 has been referenced in 4 publications.
- Liu W et al. Altered expression of voltage gated sodium channel Nav1.1 is involved in motor ability in MPTP-treated mice. Brain Res Bull 170:187-198 (2021). PubMed: 33610724
- John N et al. Comparative study of environmental pollutants bisphenol A and bisphenol S on sexual differentiation of anteroventral periventricular nucleus and spermatogenesis. Reprod Biol Endocrinol 17:53 (2019). PubMed: 31292004
- Melander T et al. Coexistence of galanin-like immunoreactivity with catecholamines, 5-hydroxytryptamine, GABA and neuropeptides in the rat CNS. J Neurosci 6:3640-54 (1986). ICC/IF ; Rat . PubMed: 2432203
- Semenenko FM et al. A monoclonal antibody against tyrosine hydroxylase: application in light and electron microscopy. J Histochem Cytochem 34:817-21 (1986). ICC/IF, IHC-P ; Rat . PubMed: 2871071