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Product Name 

U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate

Product type 

Lysates

Description 

U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate

Application notes 
(see key)

Recommended dilutions
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other

Relevance 

Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells.
The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

Storage buffer 

Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9

Form 

Liquid

Concentration 

4.160 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

 

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