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U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14903)

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    Overview

    Product name

    U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate

    General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9

    Concentration

    Concentration information loading...

    Research areas

    Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other

    Background

    Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

    Applications

    Application notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14903)

    ab14903 has not yet been referenced specifically in any publications.

    Publishing research using ab14903? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"