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Product Name
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U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate
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Product type
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Lysates
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Description
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U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate
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Application notes
(see key)
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Recommended dilutions
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other
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Relevance
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Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells.
The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.
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Storage buffer
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Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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Form
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Liquid
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Concentration
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4.160 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to U87 MG
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